Proteomic analysis of novel Cry1Ac binding proteins in Helicoverpa armigera (Hübner)

Abstract

Aminopeptidase N (APN) and cadherin‐like proteins have been previously identified as Cry1Ac‐binding proteins in Helicoverpa armigera (Hübner). In this study, a proteomic approach was used to identify novel Cry1Ac‐binding proteins in H. armigera. Brush border membrane vesicles (BBMV) of H. armigera were extracted and separated by two‐dimensional gel electrophoresis (2‐DE). Cry1Ac‐binding proteins were detected using antisera against Cry1Ac. Peptide mass fingerprinting (PMF) was used to identify Cry1Ac‐binding proteins. In total, four proteins were identified as candidate Cry1Ac‐binding proteins in H. armigera: vacuolar ATP synthase (V‐ATPase) subunit B, actin, heat shock cognate protein (HSCP), and a novel protein.