In Vivo proton spectroscopy and spectroscopic imaging of {1‐13C}‐g1ucose and its metabolic products

Abstract

Metaibolism of {1−¹³C}‐glucose was studied in situ in cat brain usinig gradient‐enhanced proton‐detected heteronuclear spectroscopy. Proton detection of {1−¹³C}‐glucose, {3−¹³C}−lactaite, 4−{¹³C}‐glutamine, 4−{¹³C}‐glutamate and the combined signals 2−{¹³C}‐glutamate/glutamine and 3−(¹³C}‐glutamate/glutamine was achieved, despite the fact that some of the associated proton resonances are close to the water signal. Two‐dimensional {¹H‐¹³C}‐spectra demonstrate the possibility of in situ spectral assignment with ¹H sensitivity and ¹³C resolution. Spectroscopic images of glucose and its metabolic products were also acquired, showing the possibility to study spatial dependence of metabolism.