Identification of a rat liver cDNA and mRNA coding for the 28 kDa gap junction protein
- 1 September 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 205 (1), 56-60
- https://doi.org/10.1016/0014-5793(86)80865-1
Abstract
By screening of a rat liver cDNA library with complex and deoxyinosine containing oligonucleotide probes a cDNA clone was isolated and shown by sequencing to code for the amino-terminal half of the rat liver 28 kDa gap junction protein. The insert hybridized to a 1.9 kb species from rat and mouse liver poly(A)+ RNA in Northern blot analysis. In embryonic mouse hepatocytes the amount of the 1.9 kb mRNA increased 3-fold between 24 and 96 h in culture. This correlates with the previously described increase of the 28 kDa gap junction protein under these conditions.Keywords
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