Vertebrate phylogeny of hydrogen sulfide vasoactivity

Abstract

Hydrogen sulfide (H₂S) is a recently identified endogenous vasodilator in mammals. In steelhead/rainbow trout ( Oncorhynchus mykiss, Osteichthyes), H₂S produces both dose-dependent dilation and a unique dose-dependent constriction. In this study, we examined H₂S vasoactivity in all vertebrate classes to determine whether H₂S is universally vasoactive and to identify phylogenetic and/or environmental trends. H₂S was generated from NaHS and examined in unstimulated and precontracted systemic and, when applicable, pulmonary arteries (PA) from Pacific hagfish ( Eptatretus stouti, Agnatha), sea lamprey ( Petromyzon marinus, Agnatha), sandbar shark ( Carcharhinus milberti, Chondrichthyes), marine toad ( Bufo marinus, Amphibia), American alligator ( Alligator mississippiensis, Reptilia), Pekin duck ( Anas platyrhynchos domesticus, Aves), and white rat ( Rattus rattus, Mammalia). In otherwise unstimulated vessels, NaHS produced 1) a dose-dependent relaxation in Pacific hagfish dorsal aorta; 2) a dose-dependent contraction in sea lamprey dorsal aorta, marine toad aorta, alligator aorta and PA, duck aorta, and rat thoracic aorta; 3) a threshold relaxation in shark ventral aorta, dorsal aorta, and afferent branchial artery; and 4) a multiphasic contraction-relaxation-contraction in the marine toad PA, duck PA, and rat PA. Precontraction of these vessels with another agonist did not affect the general pattern of NaHS vasoactivity with the exception of the rat aorta, where relaxation was now dominant. These results show that H₂S is a phylogenetically ancient and versatile vasoregulatory molecule that appears to have been opportunistically engaged to suit both organ-specific and species-specific homeostatic requirements.