Role of Purpurin as a Retinol-Binding Protein in Goldfish Retina during the Early Stage of Optic Nerve Regeneration: Its Priming Action on Neurite Outgrowth
Open Access
- 22 September 2004
- journal article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 24 (38), 8346-8353
- https://doi.org/10.1523/jneurosci.1809-04.2004
Abstract
Unlike mammals, the fish optic nerve can regenerate after injury. So far, many growth or trophic factors have been shown as an axon-regenerating molecule. However, it is totally unknown what substance regulates or triggers the activity of these factors on axonal elongation. Therefore, we constructed a goldfish retina cDNA library prepared from the retina treated with optic nerve transection 5 d previously, when it was just before regrowing optic axons after injury. A cDNA clone for goldfish purpurin for which expression was upregulated during the early stage of optic nerve regeneration was isolated from the retina cDNA library. Purpurin was discovered as a secretory retinol-binding protein in developing chicken retinas. Levels of purpurin mRNA and protein transiently increased and rapidly decreased 2–5 d and 10 d after axotomy, respectively. Purpurin mRNA was localized to the photoreceptor cells, whereas the protein was diffusely found in all of the retinal layers. A recombinant purpurin alone did not affect any change of neurite outgrowth in explant culture of the control retina, whereas a concomitant addition of the recombinant purpurin and retinol first induced a drastic enhancement of neurite outgrowth. Furthermore, the action of retinol-bound purpurin was effective only in the control (untreated) retinas but not in those primed (treated) with a previous optic nerve transection. Thus, purpurin with retinol is the first candidate molecule of priming neurite outgrowth in the early stage of optic nerve regeneration in fish.Keywords
This publication has 33 references indexed in Scilit:
- Na,K‐ATPase α3 subunit in the goldfish retina during optic nerve regenerationJournal of Neurochemistry, 2002
- Retinoid-binding proteins: mediators of retinoid actionBiochemical Journal, 2000
- Induction of ?1-tubulin gene expression during development and regeneration of the fish central nervous systemJournal of Neurobiology, 1998
- gRICH68 and gRICH70 Are 2′,3′-Cyclic-nucleotide 3′-Phosphodiesterases Induced during Goldfish Optic Nerve RegenerationPublished by Elsevier BV ,1997
- Perspectives on axonal regeneration in the mammalian CNSTrends in Neurosciences, 1994
- Digoxigenin as an Alternative Probe Labeling for In Situ HybridizationDiagnostic Molecular Pathology, 1992
- Improved method for obtaining 3-microns cryosections for immunocytochemistry.Journal of Histochemistry & Cytochemistry, 1990
- Sequence analysis, cellular localization, and expression of a neuroretina adhesion and cell survival moleculeCell, 1987
- A chick neural retina adhesion and survival molecule is a retinol-binding protein.The Journal of cell biology, 1986
- Isolation of an adhesion-mediating protein from chick neural retina adherons.The Journal of cell biology, 1985