Direct Patterning of Modified Oligonucleotides on Metals and Insulators by Dip-Pen Nanolithography
Top Cited Papers
- 7 June 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 296 (5574), 1836-1838
- https://doi.org/10.1126/science.1071480
Abstract
The use of direct-write dip-pen nanolithography (DPN) to generate covalently anchored, nanoscale patterns of oligonucleotides on both metallic and insulating substrates is described. Modification of DNA with hexanethiol groups allowed patterning on gold, and oligonucleotides bearing 5′-terminal acrylamide groups could be patterned on derivatized silica. Feature sizes ranging from many micrometers to less than 100 nanometers were achieved, and the resulting patterns exhibited the sequence-specific binding properties of the DNA from which they were composed. The patterns can be used to direct the assembly of individual oligonucleotide-modified particles on a surface, and the deposition of multiple DNA sequences in a single array is demonstrated.Keywords
This publication has 25 references indexed in Scilit:
- Protein Nanoarrays Generated By Dip-Pen NanolithographyScience, 2002
- Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical SpeciesScience, 2001
- Meniscus Force Nanografting: Nanoscopic Patterning of DNALangmuir, 2001
- Thermal-Barrier Coatings for Advanced Gas-Turbine EnginesMRS Bulletin, 2000
- A Nanoplotter with Both Parallel and Serial Writing CapabilitiesScience, 2000
- Programming the Assembly of Two- and Three-Dimensional Architectures with DNA and Nanoscale Inorganic Building Blocks,Inorganic Chemistry, 2000
- Improved Imaging of Soft Materials with Modified AFM TipsLangmuir, 1999
- "Dip-Pen" NanolithographyScience, 1999
- Using Self-Assembly To Control the Structure of DNA Monolayers on Gold: A Neutron Reflectivity StudyJournal of the American Chemical Society, 1998
- Characterization of DNA Probes Immobilized on Gold SurfacesJournal of the American Chemical Society, 1997