Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue
Open Access
- 19 March 2008
- journal article
- Published by Springer Science and Business Media LLC in BMC Cell Biology
- Vol. 9 (1), 13
- https://doi.org/10.1186/1471-2121-9-13
Abstract
Investigating the expression of candidate genes in tissue samples usually involves either immunohistochemical labelling of formalin-fixed paraffin-embedded (FFPE) sections or immunofluorescence labelling of cryosections. Although both of these methods provide essential data, both have important limitations as research tools. Consequently, there is a demand in the research community to be able to perform routine, high quality immunofluorescence labelling of FFPE tissues.Keywords
This publication has 16 references indexed in Scilit:
- Insulinlike growth factor I receptor and estrogen receptor β expressions are inversely correlated in colorectal neoplasms and affected by the insulin resistance syndromeHuman Pathology, 2007
- Phosphohistone H3 labelling for histoprognostic grading of breast adenocarcinomas and computer-assisted determination of mitotic indexJournal of Clinical Pathology, 2006
- Multiparameter Immunofluorescence on Paraffin-Embedded Tissue SectionsApplied Immunohistochemistry & Molecular Morphology, 2006
- Differential tissue distribution of the Invs gene product inversinCell and tissue research, 2005
- A new immunofluorostaining method using red fluorescence of PerCP on formalin-fixed paraffin-embedded tissuesJournal of Immunological Methods, 2004
- A novel immunohistochemical method for estimating cell cycle phase distribution in ovarian serous neoplasms: implications for the histopathological assessment of paraffin-embedded specimensBritish Journal of Cancer, 2004
- Immunofluorescence and Confocal Laser Scanning Microscopy of Chronic Myeloproliferative Disorders on Archival Formaldehyde-fixed Bone MarrowJournal of Histochemistry & Cytochemistry, 2004
- Double immunofluorescence labelling of routinely processed paraffin sectionsThe Journal of Pathology, 2000
- Loss of the Tight Junction MAGUK ZO-1 in Breast Cancer: Relationship to Glandular Differentiation and Loss of HeterozygosityThe American Journal of Pathology, 1998
- Quadruple Immunofluorescence: A Direct Visualization MethodJournal of Histochemistry & Cytochemistry, 1997