Effects of recombinant human LH and hCG on serum and urine LH and androgens in men
- 14 August 2009
- journal article
- research article
- Published by Wiley in Clinical Endocrinology
- Vol. 71 (3), 417-428
- https://doi.org/10.1111/j.1365-2265.2008.03516.x
Abstract
The administration of gonadotrophins is prohibited in sport but the effect in men of recently available recombinant hCG and LH on serum and urine concentrations of gonadotrophins and androgens has not been systematically evaluated in the antidoping context. To determine the time-course of recombinant LH (rhLH) and hCG (rhCG) on blood and urine hormone profiles in men to develop effective tests to detect rhLH and rhCG doping. Two randomized controlled studies with a 2 x 2 factorial design. Academic research centre. Healthy male volunteers aged 18-45 years. In the rhLH study, men were randomized into (i) either of two single doses of rhLH (75 IU or 225 IU), and (ii) suppression of endogenous LH and testosterone by nandrolone or no suppression. In the rhCG study, men were randomized into (i) either of two single doses of rhCG (250 or 750 microg), and (ii) suppression of endogenous LH and testosterone by nandrolone decanoate (ND) or no suppression. ND suppression comprised a single dose of 200 mg ND 3 days prior to, and in the rhCG study an additional dose 1 day after gonadotrophin injection. Serum and urine hCG, LH, T, T : LH ratio, urine epitestosterone (E) and urine T : E ratio. Neither rhLH dose produced a significant increase in serum or urine LH or T or in the T : E or T : LH ratios regardless of ND-induced suppression of endogenous LH and T. Nor did an even higher dose (750 IU) in three healthy men with unsuppressed gonadal axis. These findings were confirmed with two different commercial LH immunoassays together with adjustment for any influence of urine sediment and dilution. Both rhCG doses produced a steep, dose-proportional increase in serum and urine hCG with increases in serum and urine T and suppression of serum and urine LH, regardless of hCG dose. Serum but not urine T was lowered by ND suppression. The T : LH ratio showed a progressive increase unrelated to rhCG dose or ND suppression, whereas both rhCG and ND suppression minimally increased T : E ratio. Both rhCG doses produce a striking increase in serum hCG and T with suppression of serum LH but, at single doses up to 750 IU, rhLH has no influence on serum or urine LH or T. Effective rhLH doping, which relies on a sustained increases in endogenous T, would require much higher and more frequent daily rhLH doses. Use of LH immunoassays optimized for serum to detect rhLH doping by urine LH measurement requires more standardization and validation and, at present, is unreliable. The T : LH ratio is, however, a useful screening test for hCG doping although its utility requires further evaluation.Keywords
This publication has 65 references indexed in Scilit:
- The physiological and pharmacological basis for the ergogenic effects of androgens in elite sportsAsian Journal of Andrology, 2008
- Characterisation of the pharmacological profile of desoxymethyltestosterone (Madol), a steroid misused for dopingToxicology Letters, 2007
- The Rationale for Banning Human Chorionic Gonadotropin and Estrogen Blockers in SportJournal of Clinical Endocrinology & Metabolism, 2006
- Reproductive Hormone Reference Intervals for Healthy Fertile Young Men: Evaluation of Automated Platform AssaysJournal of Clinical Endocrinology & Metabolism, 2005
- Designer Androgens in Sport: When Too Much Is Never EnoughScience's STKE, 2004
- Tetrahydrogestrinone: discovery, synthesis, and detection in urineRapid Communications in Mass Spectrometry, 2004
- Tetrahydrogestrinone Is a Potent Androgen and ProgestinJournal of Clinical Endocrinology & Metabolism, 2004
- A Double-Blind, Placebo-Controlled, Randomized Clinical Trial of Recombinant Human Chorionic Gonadotropin on Muscle Strength and Physical Function and Activity in Older Men with Partial Age-Related Androgen DeficiencyJournal of Clinical Endocrinology & Metabolism, 2002
- Detection of norbolethone, an anabolic steroid never marketed, in athletes' urineRapid Communications in Mass Spectrometry, 2002
- Stimulation of Sperm Production by Human Luteinizing Hormone in Gonadotropin-Suppressed Normal Men*Journal of Clinical Endocrinology & Metabolism, 1984