Molecular organization of 5S rDNA in fishes of the genusBrycon
- 1 October 2001
- journal article
- Published by Canadian Science Publishing in Genome
- Vol. 44 (5), 893-902
- https://doi.org/10.1139/g01-067
Abstract
There are few reports on the genomic organization of 5S rDNA in fish species. To characterize the 5S rDNA nucleotide sequence and chromosomal localization in the Neotropical fishes of the genus Brycon, 5S rDNA copies from seven species were generated by PCR. The nucleotide sequences of the coding region (5S rRNA gene) and the nontranscribed spacer (NTS) were determined, revealing that the 5S rRNA genes were highly conserved, while the NTSs were widely variable among the species analyzed. Moreover, two classes of NTS were detected in each species, characterized by base substitutions and insertions–deletions. Using fluorescence in situ hybridization (FISH), two 5S rDNA chromosome loci that could be related to the two 5S rDNA NTS classes were observed in at least one of the species studied. 5S rDNA sequencing and chromosomal localization permitted the characterization of Brycon spp. and suggest a higher similarity among some of them. The data obtained indicate that the 5S rDNA can be an useful genetic marker for species identification and evolutionary studies.Key words: Brycon, FISH, nontranscribed spacer, nucleotide sequence, 5S rDNA.Keywords
This publication has 39 references indexed in Scilit:
- Organization of 5S rDNA in species of the fishLeporinus: two different genomic locations are characterized by distinct nontranscribed spacersGenome, 2001
- Mapping 18S ribosomal genes in fish of the genus Brycon (Characidae) by fluorescence in situ hybridization (FISH)Genetics and Molecular Biology, 2000
- Phylogenetic evaluation of 5S ribosomal RNA gene and spacer in theCallistachys group (Fabaceae:Mirbelieae)Österreichische botanische Zeitschrift, 1999
- Characterization of repetitive DNA sequences carrying 5S rDNA of the triploid ginbuna (Japanese silver crucian carp, Carassius auratus langsdorfi).Genes & Genetic Systems, 1998
- Rat 5S rDNA spacer sequences and chromosomal assignment of the genes to the extreme terminal region of chromosome 19Cytogenetic and Genome Research, 1996
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Localization and metabolic activity of ribosomal genes in Chinese hamster meiotic and mitotic chromosomesCytogenetic and Genome Research, 1980
- Adjacent repeating units of xenopus laevis 5S DNA can be heterogeneous in lengthCell, 1976
- Variation in the number of genes for rRNA among human acrocentric chromosomes: correlation with frequency of satellite associationCytogenetic and Genome Research, 1976