Challenges and Controversies to Testing for COVID-19
- 21 October 2020
- journal article
- editorial
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 58 (11)
- https://doi.org/10.1128/jcm.01695-20
Abstract
The coronavirus disease (COVID-19) pandemic has placed the clinical laboratory and testing for SARS-CoV-2 front and center in the worldwide discussion of how to end the outbreak. Clinical laboratories have responded by developing, validating, and implementing a variety of molecular and serologic assays to test for SARS-CoV-2 infection. This has played an essential role in identifying cases, informing isolation decisions, and helping to curb the spread of disease. However, as the demand for COVID-19 testing has increased, laboratory professionals have faced a growing list of challenges, uncertainties, and, in some situations, controversy, as they have attempted to balance the need for increasing test capacity with maintaining a high-quality laboratory operation. The emergence of this new viral pathogen has raised unique diagnostic questions for which there have not always been straightforward answers. In this commentary, the author addresses several areas of current debate, including (i) the role of molecular assays in defining the duration of isolation/quarantine, (ii) whether the PCR cycle threshold value should be included on patient reports, (iii) if specimen pooling and testing by research staff represent acceptable solutions to expand screening, and (iv) whether testing a large percentage of the population is feasible and represents a viable strategy to end the pandemic.Keywords
This publication has 23 references indexed in Scilit:
- Correlation of Chest CT and RT-PCR Testing for Coronavirus Disease 2019 (COVID-19) in China: A Report of 1014 CasesRadiology, 2020
- Evaluation of Saline, Phosphate-Buffered Saline, and Minimum Essential Medium as Potential Alternatives to Viral Transport Media for SARS-CoV-2 TestingJournal of Clinical Microbiology, 2020
- Report from the American Society for Microbiology COVID-19 International Summit, 23 March 2020: Value of Diagnostic Testing for SARS–CoV-2/COVID-19mBio, 2020
- Detection of SARS-CoV-2 in Different Types of Clinical SpecimensJama-Journal Of The American Medical Association, 2020
- Pooled nucleic acid testing strategy for monitoring HIV-1 treatment in resource limited settingsJournal of Clinical Virology, 2019
- A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2018 Update by the Infectious Diseases Society of America and the American Society for MicrobiologyClinical Infectious Diseases, 2018
- A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2018 Update by the Infectious Diseases Society of America and the American Society for MicrobiologyaClinical Infectious Diseases, 2018
- Occult HBV infection in HIV‐infected adults and evaluation of pooled NAT for HBVJournal of Viral Hepatitis, 2018
- Diagnosis and Treatment ofClostridium difficilein AdultsJama-Journal Of The American Medical Association, 2015
- Persistence of rhinovirus and enterovirus RNA after acute respiratory illness in childrenJournal of Medical Virology, 2004