Expression of the Wilms' tumour gene WT1 in the developing human and in paediatric renal tumours: an immunohistochemical study.
Open Access
- 1 June 1997
- journal article
- research article
- Published by BMJ in Molecular Pathology
- Vol. 50 (3), 138-144
- https://doi.org/10.1136/mp.50.3.138
Abstract
AIMS: The Wilms' tumour gene (WT1) product is expressed during the development of the urogenital system. This study was undertaken to evaluate four anti-WT1 antibodies and use the most specific one to examine the expression of WT1 in formalin fixed, paraffin wax embedded tissues from human embryos, fetuses, and paediatric renal neoplasms. METHODS: The antibodies were assessed on paraffin sections of fetal kidney and by western blotting. Immunohistochemical techniques were optimised and performed on a range of embryonic, fetal, and infant tissues from 35 days post-conception to three months of age, and on a selection of paediatric renal neoplasms. RESULTS: The antibodies tested were found to vary in their specificity. Anomalous expression in smooth muscle was seen with one batch of a commercial polyclonal antibody. WT1 protein was detected in both the metanephros and the mesonephros, the spleen, the gonads, and in the peritoneal mesothelium in fetuses. WT1 was expressed in nuclei and was strongest in the podocytes of fetal kidney. The podocytes of infant glomeruli were also positive. There was focal positive staining in Wilms' tumours, nephrogenic rests, and in a cystic partially differentiated nephroblastoma. Staining of nuclei was seen in one of two rhabdoid tumours of the kidney. No positive staining was seen in other renal tumours. CONCLUSIONS: WT1 is detected readily in formalin fixed material. There were differences in specificity between batches of the polyclonal antibodies used. The distribution of the WT1 gene product in tissues and tumours reflected previous findings with in situ hybridisation studies of WT1 mRNA.Keywords
This publication has 25 references indexed in Scilit:
- Familial Wilms' tumor: A descriptive studyMedical and Pediatric Oncology, 1996
- Subnuclear localization of WT1 in splicing or transcription factor domains is regulated by alternative splicingCell, 1995
- Towards a genetic basis for kidney developmentMechanisms of Development, 1994
- Autoregulation of the human WT1 gene promoterFEBS Letters, 1994
- Malignant rhabdoid tumor of the kidney expresses insulin–like growth factor II transcriptsPathology, 1994
- Low frequency of mutations in theWT1 coding region in Wilms' tumorGenes, Chromosomes and Cancer, 1993
- WT-1 is required for early kidney developmentCell, 1993
- Loss of heterozygosity mapping in Wilms tumor indicates the involvement of three distinct regions and a limited role for nondisjunction or mitotic recombinationGenes, Chromosomes and Cancer, 1992
- Tissue, Developmental, and Tumor-Specific Expression of Divergent Transcripts in Wilms TumorScience, 1990
- An internal deletion within an 11p13 zinc finger gene contributes to the development of Wilms' tumorCell, 1990