Use of Naturally Occurring Halloysite Nanotubes for Enhanced Capture of Flowing Cells
Open Access
- 17 June 2010
- journal article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 26 (14), 12155-12164
- https://doi.org/10.1021/la101179y
Abstract
The development of individualized treatments for cancer can be facilitated by more efficient methods for separating cancer cells from patient blood in such a way that they remain viable for live cell assays. We have previously shown that immobilized P-selectin protein can be used on the inner surface of a microscale flow system to induce leukemic cells and leukocytes to roll at different velocities and relative fluxes, thereby creating a means for rapid cell fractionation without inflicting cellular damage. In this study, we explore a method to more efficiently capture leukemic and epithelial cancer cells from flow by altering the nanoscale topography of the inner surface of P-selectin-coated microtubes. This functionalized topography is achieved by attaching naturally occurring halloysite nanotubes to the microtube surface via a monolayer of poly-l-lysine), followed by functionalization with recombinant human selectin protein. We have found that halloysite nanotube coatings promote increased capture of leukemic cells and have determined the key parameters for controlling cell capture under flow: halloysite content and selectin density. Ultimately, selectin-functionalized nanotube coatings should provide a means for enhanced cancer cell isolation from whole blood and other mixtures of cells.Keywords
This publication has 35 references indexed in Scilit:
- Delivery of apoptotic signal to rolling cancer cells: A novel biomimetic technique using immobilized TRAIL and E‐selectinBiotechnology & Bioengineering, 2008
- Capture and enrichment of CD34‐positive haematopoietic stem and progenitor cells from blood circulation using P‐selectin in an implantable deviceBritish Journal of Haematology, 2008
- P-Selectin–Coated Microtube for Enrichment of CD34+ Hematopoietic Stem and Progenitor Cells from Human Bone MarrowClinical Chemistry, 2008
- Circulating tumor cells (CTC) detection: Clinical impact and future directionsCancer Letters, 2007
- Cancer Invasion and MetastasisOncology, 2005
- DETECTION OF DISSEMINATED TUMOR CELLS IN PERIPHERAL BLOODCritical Reviews in Clinical Laboratory Sciences, 2005
- Tumor Cells Circulate in the Peripheral Blood of All Major Carcinomas but not in Healthy Subjects or Patients With Nonmalignant DiseasesClinical Cancer Research, 2004
- Frequent EpCam protein expression in human carcinomasHuman Pathology, 2004
- White Blood Cell Count Is Associated With Macro- and Microvascular Complications in Chinese Patients With Type 2 DiabetesDiabetes Care, 2004
- Mosaic blood vessels in tumors: Frequency of cancer cells in contact with flowing bloodProceedings of the National Academy of Sciences of the United States of America, 2000