Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays
Open Access
- 23 April 2009
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 10 (4), 1930-1941
- https://doi.org/10.3390/ijms10041930
Abstract
Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.Keywords
This publication has 35 references indexed in Scilit:
- A 128 $\times$ 128 Single-Photon Image Sensor With Column-Level 10-Bit Time-to-Digital Converter ArrayIEEE Journal of Solid-State Circuits, 2008
- Semiconductor Quantum Dots for BioanalysisAngewandte Chemie-International Edition, 2008
- Toward Single-metal-ion Sensing by Förster Resonance Energy TransferAnnals of the New York Academy of Sciences, 2008
- Quantum dot-based immunosensor for the detection of prostate-specific antigen using fluorescence microscopyTalanta, 2007
- Luminescence and Stability of Aqueous Thioalkyl Acid Capped CdSe/ZnS Quantum Dots Correlated to Ligand IonizationChemphyschem, 2007
- Interaction between Presenilin 1 and Ubiquilin 1 as Detected by Fluorescence Lifetime Imaging Microscopy and a High-throughput Fluorescent Plate ReaderPublished by Elsevier BV ,2006
- Quantum dots-based reverse phase protein microarrayTalanta, 2005
- Experimental design for three-color and four-color gene expression microarraysBioinformatics, 2005
- Quantum dot bioconjugates for imaging, labelling and sensingNature Materials, 2005
- Ensemble and single particle photophysical properties (two‐photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cellsMicroscopy Research and Technique, 2004