Screening of a fungus capable of powerful and selective delignification on wheat straw
- 1 November 2008
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 47 (5), 415-420
- https://doi.org/10.1111/j.1472-765x.2008.02447.x
Abstract
To screen and characterize a novel fungus with powerful and selective delignification capability on wheat straw. A fungus capable of efficient delignification under solid-state fermentation (SSF) conditions on wheat straw was screened. After 5 days of incubation, 13.07% of the lignin was removed by fungal degradation, and 7.62% of the holocellulose was lost. Furthermore, 46.53% of the alkali lignin was removed after 2 days of liquid fermentation. The fungus was identified as Fusarium concolor based on its morphology and an analysis of its 18S rDNA gene sequence. The molecular weight distribution of lignin was evaluated by gel permeation chromatography. Enzyme assay indicated that the fungus produced laccase, cellobiose dehydrogenase, xylanase and cellulase during the incubation period. Intracellular lignin peroxidase, manganese peroxidase and laccase were produced during liquid fermentation. We have successfully screened a fungus, F. concolor, which can efficiently degrade the lignin of wheat straw, with slight damage to the cellulose, after 5 days of SSF. The newly isolated strain could be used in pretreatment of lignocellulose materials prior to biopulping, bioconversion into fuel and substrates for the chemical industry.Keywords
This publication has 29 references indexed in Scilit:
- Chemical investigation of wood tree species in a temperate forest east-northern RomaniaBioResources, 2007
- Two isolates ofFusarium proliferatumfrom different habitats and global locations have similar abilities to degrade ligninFEMS Microbiology Letters, 2005
- Continuous enzymatic regeneration of redox mediators used in biotransformation reactions employing flavoproteinsJournal of Molecular Catalysis B: Enzymatic, 2001
- Biological upgrading of wheat straw through solid-state fermentation with Streptomyces cyaneus.Applied Microbiology and Biotechnology, 2000
- A cellulase-poor, thermostable, alkalitolerant xylanase produced by Bacillus circulans AB 16 grown on rice straw and its application in biobleaching of eucalyptus pulpBioresource Technology, 2000
- Transformation of wheat straw in the course of solid-state fermentation by four ligninolytic basidiomycetesEnzyme and Microbial Technology, 1999
- Short communications: Effect of water activity on cell growth and phenol oxidase production by Streptomyces cyneus var. viridochromogenes in surface cultureWorld Journal of Microbiology and Biotechnology, 1996
- Cellobiose dehydrogenases of Sporotrichum (Chrysosporium) thermophileEuropean Journal of Biochemistry, 1991
- Microbial and Enzymatic Degradation of Wood and Wood ComponentsSpringer Series in Wood Science, 1990
- Lignin BiodegradationCritical Reviews in Biotechnology, 1987