Recombinant Avian Infectious Bronchitis Virus Expressing a Heterologous Spike Gene Demonstrates that the Spike Protein Is a Determinant of Cell Tropism
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Open Access
- 15 August 2003
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (16), 9084-9089
- https://doi.org/10.1128/jvi.77.16.9084-9089.2003
Abstract
A recombinant infectious bronchitis virus (IBV), BeauR-M41(S), was generated using our reverse genetics system (R. Casais, V. Thiel, S. G. Siddell, D. Cavanagh, and P. Britton, J. Virol. 75: 12359-12369, 2001), in which the ectodomain region of the spike gene from IBV M41-CK replaced the corresponding region of the IBV Beaudette genome. BeauR-M41(S) acquired the same cell tropism phenotype as IBV M41-CK in four different cell types, demonstrating that the IBV spike glycoprotein is a determinant of cell tropism.This publication has 22 references indexed in Scilit:
- Coronaviruses from pheasants ( Phasianus colchicus ) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeysAvian Pathology, 2002
- Protection of chickens against renal damage caused by a nephropathogenic infectious bronchitis virusAvian Pathology, 2001
- Detection of a coronavirus from turkey poults in Europe genetically related to infectious bronchitis virus of chickensAvian Pathology, 2001
- A nomenclature for avian coronavirus isolates and the question of species statusAvian Pathology, 2001
- Murine Coronavirus Spike Protein Determines the Ability of the Virus To Replicate in the Liver and Cause HepatitisJournal of Virology, 2001
- Assembly of Spikes into Coronavirus Particles Is Mediated by the Carboxy-Terminal Domain of the Spike ProteinJournal of Virology, 2000
- Coronavirus motifNature, 1991
- Evidence for a coiled-coil structure in the spike proteins of coronavirusesJournal of Molecular Biology, 1987
- Taxonomic studies on strains of avian infectious bronchitis virus using neutralisation tests in tracheal organ culturesArchiv für die gesamte Virusforschung, 1979
- Studies on avian infectious bronchitis virus (IBV)Archiv für die gesamte Virusforschung, 1979