Proteolytic enzyme activity of juvenile Asian sea bass,Lates calcarifer(Bloch), is increased with protein intake
- 18 June 2002
- journal article
- Published by Hindawi Limited in Aquaculture Research
- Vol. 33 (8), 569-574
- https://doi.org/10.1046/j.1365-2109.2002.00682.x
Abstract
The effect of high dietary protein intake on proteolytic enzyme activity of feeding juvenile Asian sea bass, Lates calcarifer (Bloch) was studied. Ninety fish [mean body weight ± standard error (SE) 304.62 ± 34.84 g] were randomly assigned to two dietary treatments, each with three replicates. In treatment 1, fish were fed by-catch (Thunnus albacares) and in treatment 2, a formulated diet containing 50% protein. Proteolytic enzyme activity was determined in pyloric caecae and intestine at day 0, 7, 15, and 30. Initial proteolytic enzyme activity in sea bass ranged from 174 to 232 azocasein units (UAC.) per mg of protein. After 7 days there was no significant difference in proteolytic enzyme activity of fish fed the two diets. However, a marked increase was observed in fish fed the formulated diet at day 15. After 30 days, the proteolytic enzyme activity in fish fed the formulated diet was threefold higher than that in fish fed the by-catch diet. Fish fed the formulated diet had significantly higher total protein intake at day 7 than did fish fed by-catch. Thereafter, a twofold weekly increase in the total protein intake was observed in both fish fed the by-catch and formulated diets until day 30. These results suggest that a high dietary protein intake induces increased proteolytic enzyme activity in Asian sea bassKeywords
This publication has 18 references indexed in Scilit:
- Protein digestion in penaeid shrimp: digestive proteinases, proteinase inhibitors and feed digestibilityAquaculture, 2000
- Replacement of fish meal with various types of soybean products in diets for the Asian seabass, Lates calcariferAquaculture, 1998
- Adaptation of trypsin, chymotrypsin and α-amylase to casein level and protein source in Penaeus vannamei (Crustacea Decapoda)Journal of Experimental Marine Biology and Ecology, 1997
- Sunflower meal compared with soybean meals as partial substitutes for fish meal in rainbow trout (Oncorhynchus mykiss) diets: protein and energy utilizationAquaculture, 1994
- Evaluation of soybean meal, spirulina meal and chicken offal meal as protein sources for silver seabream (Rhabdosargus sarba) fingerlingsAquaculture, 1994
- Binding of soybean agglutinin to small intestinal brush border membranes and brush border membrane enzyme activities in Atlantic salmon (Salmo salar)Aquaculture, 1990
- Studies on proteinases from the digestive organs of sardine. I. Purification and characterization of three alkaline proteinases from the pyloric caecaBiochimica et Biophysica Acta (BBA) - Enzymology, 1981
- Digestive enzymes of the pyloric caeca and of their associated mesentery in the cod (Gadus morhua)Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1973
- Studies on Proteinase in the Pyloric Caeca of Fishes-IIINIPPON SUISAN GAKKAISHI, 1971
- STUDIES ON THE DIGESTIVE ENZYMES OF RAINBOW TROUT-IINIPPON SUISAN GAKKAISHI, 1960