Use of antisense RNA technology to study pectin degradation in tomato fruit
- 1 April 1992
- journal article
- research article
- Published by Informa UK Limited in New Zealand Journal of Crop and Horticultural Science
- Vol. 20 (2), 119-124
- https://doi.org/10.1080/01140671.1992.10421904
Abstract
Antisense RNA technology is a powerful tool to study the metabolic role of enzymes and to produce commercially desirable traits in food crops. This is demonstrated here for the study of pectin degradation during tomato (Lycopersicon esculentum Mill) fruit ripening. Two enzymes—polygalacturonase (PG) and pectinesterase (PE), have been down regulated using this technique. Reduced PG prevented pectin depolymerisation during ripening and resulted in fruit with improved storage and transport characteristics. The reduction in PE activity resulted in increased pectin esterification throughout ripening but had no apparent effect on fruit physiology. All other aspects of pectin metabolism tested were unaffected in these transgenic fruit.Keywords
This publication has 25 references indexed in Scilit:
- REGULATION OF GENE EXPRESSION IN TRANSGENIC TOMATO PLANTS BY ANTISENSE RNA AND RIPENING-SPECIFIC PROMOTERSPublished by Elsevier BV ,1990
- Expression of a chimeric polygalacturonase gene in transgenic rin (ripening inhibitor) tomato fruit results in polyuronide degradation but not fruit softening.Plant Cell, 1989
- The tomato polygalacturonase gene and ripening-specific expression in transgenic plantsPlant Molecular Biology, 1988
- Molecular cloning of tomato fruit polygalacturonase: Analysis of polygalacturonase mRNA levels during ripeningProceedings of the National Academy of Sciences of the United States of America, 1986
- Sequencing and identification of a cDNA done for tomato polygalacturonaseNucleic Acids Research, 1986
- Fractionation and partial characterization of cell walls from normal and non‐ripening mutant tomato fruitPhysiologia Plantarum, 1984
- Ultrastructure of Tomato Fruit Ripening and the Role of Polygalacturonase Isoenzymes in Cell Wall DegradationPlant Physiology, 1983
- Timing of ethylene and polygalacturonase synthesis in relation to the control of tomato fruit ripeningPlanta, 1983
- Polygalacturonase in normal and abnormal tomato fruitBiochemical Journal, 1964
- Pectinesterase in normal and abnormal tomato fruitBiochemical Journal, 1963