Detection of adenoviruses in stool specimens by nucleic acid spot hybridization

Abstract

Nucleic acid hybridization was used for the detection of adenovirus DNA in stool specimens, and the results were compared with those obtained by a radioimmunoassay (RIA) for adenovirus hexon antigen. DNA from 40 specimens, 18 of which were positive by RIA, were spotted onto nitrocellulose filters and analyzed by hybridization using radioactively labeled adenovirus‐2 DNA or a cloned DNA fragment from enteric adenovirus‐41 as probes. With the adenovirus‐2 DNA probe, 15 of the 18 RIA‐positive specimens were also positive in the hybridization assay, and one of the RIA negative specimens was also scored as positive. The cloned adenovirus‐41 fragment gave a positive signal with five specimens, all of which were also detected with the adenovirus‐2 DNA probe. The results show that hybridization is an alternative method for detection of adenovirus in stool specimens. The sensitivity of the assay is comparable to that of the RIA.