Cauliflower Mosaic Virus: an Improved Purification Procedure and Some Properties of the Virus Particles
- 31 March 1976
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 31 (1), 93-100
- https://doi.org/10.1099/0022-1317-31-1-93
Abstract
A new procedure for the purification of cauliflower mosaic virus (CaMV) which involves the use of Triton X-100 and urea results in higher and more reliable yields. CaMV particles are 208S, have a diffusion coefficient of 0.75 .times. 10-7 cm2/s, contain 17% nucleic acid and have a MW of 22.8 .times. 106. Virus precipitated by Mg2+, low pH or ethanol at moderate salt concentrations, or by polyethylene glycol, is not easily resuspended.This publication has 3 references indexed in Scilit:
- The use of bentonite in the purification of plant virusesVirology, 1965
- Optical methods for measuring nucleoprotein and nucleic acid concentrationsArchives of Biochemistry and Biophysics, 1957
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956