Label-free integrative pharmacology on-target of drugs at the β2-adrenergic receptor
Open Access
- 7 July 2011
- journal article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 1 (1), 33
- https://doi.org/10.1038/srep00033
Abstract
We describe a label-free integrative pharmacology on-target (iPOT) method to assess the pharmacology of drugs at the β2-adrenergic receptor. This method combines dynamic mass redistribution (DMR) assays using an array of probe molecule-hijacked cells with similarity analysis. The whole cell DMR assays track cell system-based, ligand-directed, and kinetics-dependent biased activities of the drugs, and translates their on-target pharmacology into numerical descriptors which are subject to similarity analysis. We demonstrate that the approach establishes an effective link between the label-free pharmacology and in vivo therapeutic indications of drugs.This publication has 33 references indexed in Scilit:
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