Multiplexed optical pathogen detection with lab‐on‐a‐chip devices
- 14 April 2009
- journal article
- review article
- Published by Wiley in Journal of Biophotonics
- Vol. 2 (4), 199-211
- https://doi.org/10.1002/jbio.200910009
Abstract
Infectious diseases are still a main cause of human morbidity and mortality. Advanced diagnostics is considered to be a key driver to improve the respective therapeutic outcome. The main factors influencing the impact of diagnostics include: assay speed, availability, information content, in‐vitro diagnostics and cost, for which molecular assays are providing the most promising opportunities. Miniaturisation and integration of assay steps into lab‐on‐a‐chip devices has been described as an appropriate way to speed up assay time and make assays available onsite at competitive costs. As meaningful assays for infectious diseases need to include a whole range of clinical relevant information about the pathogen, multiplexed functionality is often required for which optical transduction is particularly well suited. The aim of this review is to assess existing developments in this field and to give an outlook on future requirements and solutions.Keywords
This publication has 86 references indexed in Scilit:
- Perspective on Optical Biosensors and Integrated Sensor SystemsAnalytical Chemistry, 2008
- Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of bloodNature Biotechnology, 2008
- Towards non- and minimally instrumented, microfluidics-based diagnostic devicesLab on a Chip, 2008
- Detecting bacteria and determining their susceptibility to antibiotics by stochastic confinement in nanoliter droplets using plug-based microfluidicsLab on a Chip, 2008
- Resequencing microarray probe design for typing genetically diverse viruses: human rhinoviruses and enterovirusesBMC Genomics, 2008
- Polymer Microfluidic Chips with Integrated Waveguides for Reading MicroarraysAnalytical Chemistry, 2007
- A fully integrated microfluidic genetic analysis system with sample-in–answer-out capabilityProceedings of the National Academy of Sciences of the United States of America, 2006
- Rapid and simple detection of food poisoning bacteria by bead assay with a microfluidic chip-based systemJournal of Microbiological Methods, 2006
- A DNA biochip for on-the-spot multiplexed pathogen identificationNucleic Acids Research, 2006
- Development of a DNA Microarray for Detection and Identification of Fungal Pathogens Involved in Invasive MycosesJournal of Clinical Microbiology, 2005