Glucose decreases respiratory control ratio in EL‐4 tumor cells
- 23 November 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 313 (2), 126-128
- https://doi.org/10.1016/0014-5793(92)81427-n
Abstract
EL-4 ascites thymoma cells are shown to have high aerobic glycolysis and decreased Pasteur effect. At the same time, glucose produces a much smaller inhibitory effect on cell respiration (Crabtree effect) than in Ehrlich ascites carcinoma (EAC) cells. In intact EL-4 cells, the respiratory control ratio (RCR) was found to be 6.2 with endogenous substrates and 8.0 with glutamine. Glucose decreased the RCR to 3.2, by stimulating, the state 4 respiration. In rat thymocytes and EAC cells, such an effect of glucose was absent (RCR of 7.0 and 7.2, respectively). It is suggested that in EL-4 tumor cells, the high aerobic glycolysis and small Crabtree effect may be due to glucose-induced ‘uncoupling’ of oxidation and phosphorylationKeywords
This publication has 10 references indexed in Scilit:
- Functional significance of mitochondrial bound hexokinase in tumor cell metabolism. Evidence for preferential phosphorylation of glucose by intramitochondrially generated ATP.Journal of Biological Chemistry, 1988
- Measurement of ‘in situ’ mitochondrial membrane potential in Ehrlich ascites tumor cells during aerobic glycolysisBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1988
- Mechanisms of oxidant-mediated cell injury. The glycolytic and mitochondrial pathways of ADP phosphorylation are major intracellular targets inactivated by hydrogen peroxide.Journal of Biological Chemistry, 1988
- Expression of the v-src or v-fps oncogene increases fructose 2,6-bisphosphate in chick-embryo fibroblasts. Novel mechanism for the stimulation of glycolysis by retrovirusesBiochemical Journal, 1986
- The role of ATPase in glycolysis of Ehrlich ascites tumor cells.Journal of Biological Chemistry, 1983
- Oxidative phosphorylation and ATPase activities of human tumor mitochondriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1980
- Mitochondrial ATPase activities of hepatoma BW7756 and ascites tumor cells. Influence of MG2+ ions, free fatty acids, and uncouplers.Journal of Biological Chemistry, 1978
- Tumor Mitochondria and the Bioenergetics of Cancer CellsProgress in experimental tumor research, 1977
- On the mechanism of the crabtree effect in mouse ascites tumor cellsJournal of Cellular Physiology, 1977
- Hexokinase Inhibition after the Addition of Glucose to Ehrlich-Lettré Ascites Tumor Cells under AerobiosisJournal of Biological Chemistry, 1968