TEAD1-dependent expression of the FoxO3a gene in mouse skeletal muscle
Open Access
- 7 January 2011
- journal article
- Published by Springer Science and Business Media LLC in BMC Molecular Biology
- Vol. 12 (1), 1
- https://doi.org/10.1186/1471-2199-12-1
Abstract
TEAD1 (TEA domain family member 1) is constitutively expressed in cardiac and skeletal muscles. It acts as a key molecule of muscle development, and trans-activates multiple target genes involved in cell proliferation and differentiation pathways. However, its target genes in skeletal muscles, regulatory mechanisms and networks are unknown. In this paper, we have identified 136 target genes regulated directly by TEAD1 in skeletal muscle using integrated analyses of ChIP-on-chip. Most of the targets take part in the cell process, physiology process, biological regulation metabolism and development process. The targets also play an important role in MAPK, mTOR, T cell receptor, JAK-STAT, calcineurin and insulin signaling pathways. TEAD1 regulates foxo3a transcription through binding to the M-CAT element in foxo3a promoter, demonstrated with independent ChIP-PCR, EMSA and luciferase reporter system assay. In addition, results of over-expression and inhibition experiments suggest that foxo3a is positively regulated by TEAD1. Our present data suggests that TEAD1 plays an important role in the regulation of gene expression and different signaling pathways may co-operate with each other mediated by TEAD1. We have preliminarily concluded that TEAD1 may regulate FoxO3a expression through calcineurin/MEF2/NFAT and IGF-1/PI3K/AKT signaling pathways in skeletal muscles. These findings provide important clues for further analysis of the role of FoxO3a gene in the formation and transformation of skeletal muscle fiber types.Keywords
This publication has 27 references indexed in Scilit:
- Reciprocal Regulation of MicroRNA-1 and Insulin-Like Growth Factor-1 Signal Transduction Cascade in Cardiac and Skeletal Muscle in Physiological and Pathological ConditionsCirculation, 2009
- Evidence of MyomiR network regulation of β-myosin heavy chain gene expression during skeletal muscle atrophyPhysiological Genomics, 2009
- Systematic and integrative analysis of large gene lists using DAVID bioinformatics resourcesNature Protocols, 2008
- Porcine TEF1 and RTEF1: Molecular characterization and association analyses with growth traitsComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2008
- LongSAGE analysis of skeletal muscle at three prenatal stages in Tongcheng and Landrace pigsGenome Biology, 2007
- TEF-1 and C/EBPβ are major p38α MAPK-regulated transcription factors in proliferating cardiomyocytesBiochemical Journal, 2006
- Transcription cofactor Vgl‐2 is required for skeletal muscle differentiationgenesis, 2004
- The IGF-1/PI3K/Akt Pathway Prevents Expression of Muscle Atrophy-Induced Ubiquitin Ligases by Inhibiting FOXO Transcription FactorsMolecular Cell, 2004
- TEF-1 and MEF2 transcription factors interact to regulate muscle-specific promotersBiochemical and Biophysical Research Communications, 2002
- Identification of a Murine TEF-1-related Gene Expressed after Mitogenic Stimulation of Quiescent Fibroblasts and during Myogenic DifferentiationOnline Journal of Public Health Informatics, 1996