Intrahepatic Induction of Alpha/Beta Interferon Eliminates Viral RNA-Containing Capsids in Hepatitis B Virus Transgenic Mice
- 1 May 2000
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 74 (9), 4165-4173
- https://doi.org/10.1128/jvi.74.9.4165-4173.2000
Abstract
We have previously shown that hepatitis B virus (HBV) replication is abolished in the liver of HBV transgenic mice by stimuli that induce alpha/beta interferon (IFN-α/β) in the liver. The present study was done to identify the step(s) in HBV replication that is affected by this cytokine in transgenic mice treated with the IFN-α/β inducer polyinosinic-polycytidylic acid [poly(I-C)]. Here we show that the pool of cytoplasmic HBV pregenomic RNA (pgRNA)-containing capsids is reduced 10-fold within 9 h after poly(I-C) administration, while there is no change in the abundance of HBV mRNA or in the translational status of cytoplasmic HBV transcripts. In addition, we show that the pool of HBV DNA-containing capsids is not reduced to the same degree until at least 15 h posttreatment, and we show that virus export is not accelerated and the half-life of virions in the serum is unchanged. These results indicate that IFN-α/β triggers intracellular events that either inhibit the assembly of pgRNA-containing capsids or accelerate their degradation, and that maturation and secretion of virus is responsible for clearance of HBV capsids and their cargo of replicative intermediates from the cytoplasm of the hepatocyte.This publication has 23 references indexed in Scilit:
- Cytokine-induced viral purging — role in viral pathogenesisCurrent Opinion in Microbiology, 1999
- Viral Clearance Without Destruction of Infected Cells During Acute HBV InfectionScience, 1999
- HOW CELLS RESPOND TO INTERFERONSAnnual Review of Biochemistry, 1998
- Hepatitis B virus replication-an updateJournal of Viral Hepatitis, 1996
- Hepatitis B Virus ImmunopathogenesisAnnual Review of Immunology, 1995
- Proteins associated with the messenger ribonucleoprotein particle for the estrogen-regulated apolipoprotein II mRNABiochemistry, 1992
- Constitutive expression of (2′–5′) oligo A synthetase confers resistance to picornavirus infectionNature, 1987
- Immunohistochemical detection of proliferating cells in vivo.Journal of Histochemistry & Cytochemistry, 1987
- Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediateCell, 1982
- Inhibition of viral protein synthesis in monkey cells treated with interferon late in simian virus 40 lytic cycleCell, 1977