Role of Heat-Stable Enterotoxins in the Induction of Early Immune Responses in Piglets after Infection with Enterotoxigenic Escherichia coli
Open Access
- 17 July 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (7), e41041
- https://doi.org/10.1371/journal.pone.0041041
Abstract
Enterotoxigenic Escherichia coli (ETEC) strains that produce heat-stable (ST) and/or heat - labile (LT) enterotoxins are cause of post – weaning diarrhea in piglets. However, the relative importance of the different enterotoxins in host immune responses against ETEC infection has been poorly defined. In the present study, several isogenic mutant strains of an O149:F4ac+, LT+ STa+ STb+ ETEC strain were constructed that lack the expression of LT in combination with one or both types of ST enterotoxins (STa and/or STb). The small intestinal segment perfusion (SISP) technique and microarray analysis were used to study host early immune responses induced by these mutant strains 4 h after infection in comparison to the wild type strain and a PBS control. Simultaneously, net fluid absorption of pig small intestinal mucosa was measured 4 h after infection, allowing us to correlate enterotoxin secretion with gene regulation. Microarray analysis showed on the one hand a non-toxin related general antibacterial response comprising genes such as PAP, MMP1 and IL8. On the other hand, results suggest a dominant role for STb in small intestinal secretion early after post-weaning infection, as well as in the induced innate immune response through differential regulation of immune mediators like interleukin 1 and interleukin 17.This publication has 76 references indexed in Scilit:
- Associations between Mucosal Innate and Adaptive Immune Responses and Resolution of Diarrheal Pathogen InfectionsInfection and Immunity, 2010
- Escherichia coli Constructs Expressing Human or Porcine Enterotoxins Induce Identical Diarrheal Diseases in a Piglet Infection ModelApplied and Environmental Microbiology, 2008
- Comparison of the Contributions of Heat-Labile Enterotoxin and Heat-Stable Enterotoxin b to the Virulence of Enterotoxigenic Escherichia coli in F4ac Receptor-Positive Young PigsInfection and Immunity, 2008
- Broad Up-Regulation of Innate Defense Factors during Acute CholeraInfection and Immunity, 2007
- Interleukin-23 drives innate and T cell–mediated intestinal inflammationThe Journal of Experimental Medicine, 2006
- Comparison of Virulence Gene Profiles of Escherichia coli Strains Isolated from Healthy and Diarrheic SwineApplied and Environmental Microbiology, 2006
- Significance of Heat-Stable and Heat-Labile Enterotoxins in Porcine Colibacillosis in an Additive Model for Pathogenicity StudiesInfection and Immunity, 2006
- Matrix metalloproteinases as modulators of inflammation and innate immunityNature Reviews Immunology, 2004
- Gene Expression Omnibus: NCBI gene expression and hybridization array data repositoryNucleic Acids Research, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001