Guanosine Quadruplexes in Solution: A Small-Angle X-Ray Scattering Analysis of Temperature Effects on Self-Assembling of Deoxyguanosine Monophosphate
Open Access
- 1 January 2010
- journal article
- Published by Hindawi Limited in Journal of Nucleic Acids
- Vol. 2010, 1-10
- https://doi.org/10.4061/2010/472478
Abstract
We investigated quadruplex formation in aqueous solutions of-deoxyriboguanosine-monophosphate, d(pG), which takes place in the absence of the covalent axial backbone. A series of in-solution small angle X-ray scattering experiments on d(pG) have been performed as a function of temperature in the absence of excess salt, at a concentration just above the critical one at which self-assembling occurs. A global fit approach has been used to derive composition and size distribution of the scattering particles as a function of temperature. The obtained results give thermodynamical justification for the observed phase-behavior, indicating that octamer formation is essential for quadruplex elongation. Our investigation shows that d(pG) quadruplexes are very suitable to assess the potential of G-quadruplex formation and to study the self-assembling thermodynamics.
Keywords
Funding Information
- Ministero dell’Istruzione, dell’Università e della Ricerca
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