High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry
- 28 February 2008
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Protocols
- Vol. 3 (3), 486-504
- https://doi.org/10.1038/nprot.2007.500
Abstract
Flow injection electrospray–mass spectrometry (FIE–MS) is finding utility as a first-pass metabolite fingerprinting tool in many research fields. We provide a protocol that has proved reliable in large-scale research projects involving diverse sample matrices originating from plants, microbes and mammalian biofluids. Using Brachypodium leaf material as an example matrix all steps are summarized from sample extraction to data quality assessment. Alternative procedures for dealing with other common matrices such as bloods and urine are included. With little sample pretreatment, no chromatography and instrument cycle times of 1,000 samples per week. Analysis of a typical batch of 240 samples (including first-pass data analysis) can be accomplished within 48 h.Keywords
This publication has 58 references indexed in Scilit:
- Rice blast infection of Brachypodium distachyon as a model system to study dynamic host/pathogen interactionsNature Protocols, 2008
- Preprocessing, classification modeling and feature selection using flow injection electrospray mass spectrometry metabolite fingerprint dataNature Protocols, 2008
- Regulation of yeast oscillatory dynamicsProceedings of the National Academy of Sciences, 2007
- Predicting interpretability of metabolome models based on behavior, putative identity, and biological relevance of explanatory signalsProceedings of the National Academy of Sciences of the United States of America, 2006
- The ion suppression phenomenon in liquid chromatography–mass spectrometry and its consequences in the field of residue analysisAnalytica Chimica Acta, 2005
- Functional genomics via metabolic footprinting: monitoring metabolite secretion by Escherichia coli tryptophan metabolism mutants using FT–IR and direct injection electrospray mass spectrometryComparative and Functional Genomics, 2003
- Ion suppression in the determination of clenbuterol in urine by solid‐phase extraction atmospheric pressure chemical ionisation ion‐trap mass spectrometryRapid Communications in Mass Spectrometry, 2002
- Nontargeted Metabolome Analysis by Use of Fourier Transform Ion Cyclotron Mass SpectrometryOMICS: A Journal of Integrative Biology, 2002
- From genomics to proteomics: techniques and applications in cancer researchTrends in Cell Biology, 2001
- Using direct electrospray mass spectrometry in taxonomy and secondary metabolite profiling of crude fungal extractsJournal of Microbiological Methods, 1996