The effect of myocardial infarction on the synthesis, concentration and receptor expression of endogenous melatonin
- 13 December 2006
- journal article
- Published by Wiley in Journal of Pineal Research
- Vol. 42 (3), 254-260
- https://doi.org/10.1111/j.1600-079x.2006.00413.x
Abstract
We examined the time course of changes in the synthesis and levels of endogenous melatonin and in the expression of MT1 and MT2 melatonin receptors 1 day, 2 and 4 wk after myocardial infarction (MI) in rats. MI was produced by ligation of the left anterior descending coronary artery. Transthoracic echocardiography was performed to characterize structural and functional changes after MI. mRNA levels were measured by real‐time quantitative reverse transcription‐polymerase chain reaction and proteins by Western blotting. One day after infarction, MI rats had 4.3 times (P < 0.001) higher pineal melatonin synthesis, than sham‐operated animals, which was associated with the increased concentration of melatonin in plasma (P < 0.001) and left ventricle (LV) (P = 0.01). The amount of MT1 receptor protein decreased significantly in MI LVs compared with control LVs 1 day after infarction (P < 0.01), followed by recovery during the next 2 wk. Furthermore, the expression of MT1 receptor mRNA of the MI LVs was elevated 2 wk after infarction (P < 0.01) compared with control LVs. The amount of MT2 receptor proteins in MI LVs was higher than in sham‐operated LVs 1 day (P < 0.05) and 4 wk (P < 0.01) after MI. In conclusion, melatonin synthesis in the pineal gland increased rapidly in response to the MI, supporting an important role for endogenous melatonin in protecting the heart after MI. The observed changes in the expression of MT1 and MT2 receptors suggest that melatonin receptors may be involved in mediating, at least, in part, the protective effects of melatonin in the heart after infarction.Keywords
This publication has 28 references indexed in Scilit:
- A proposed mechanism to explain the stimulatory effect of melatonin on antioxidative enzymesJournal of Pineal Research, 2005
- What constitutes a physiological concentration of melatonin?Journal of Pineal Research, 2002
- Effects of physiological and pharmacological concentrations of melatonin on ischemia–reperfusion arrhythmias in rats: can the incidence of sudden cardiac death be reduced?Journal of Pineal Research, 2002
- Cellular and Molecular Actions of Melatonin as an AntioxidantPublished by S. Karger AG ,1997
- Brain natriuretic peptide in plasma, atria, and ventricles of vasopressin- and phenylephrine-infused conscious ratsEndocrinology, 1994
- Pineal gland interface between the photoperiodic environment and the endocrine systemTrends in Endocrinology & Metabolism, 1991
- Presence of immunoreactive melatonin in different tissues of the pigeon (Columba livia)General and Comparative Endocrinology, 1985
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences of the United States of America, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970