Evaluation of Analytical Methods for Carotenoid Extraction from Biofortified Maize (Zea mays sp.)
- 22 September 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 54 (21), 7992-7997
- https://doi.org/10.1021/jf062256f
Abstract
Biofortification of maize with beta-carotene has the potential to improve vitamin A status in vitamin A deficient populations where maize is a staple crop. Accurate assessment of provitamin A carotenoids in maize must be performed to direct breeding efforts. The objective was to evaluate carotenoid extraction methods and determine essential steps for use in countries growing biofortified maize. The most reproducible method based on coefficient of variation and extraction efficiency was a modification of Kurilich and Juvik (1999). Heat and saponification are required to release carotenoids from biofortified maize and remove oils interfering with chromatographic analysis. For maize samples with high oil content, additional base may be added to ensure complete saponification without compromising results. Degradation of internal standard before carotenoids were released from the maize matrix required the addition of internal standard after heating to prevent overestimation of carotenoids. This modified method works well for lutein, zeaxanthin, beta-cryptoxanthin, alpha-carotene, and beta-carotene.This publication has 10 references indexed in Scilit:
- Twice the Amount of α-Carotene Isolated from Carrots Is as Effective as β-Carotene in Maintaining the Vitamin A Status of Mongolian GerbilsJournal of Nutrition, 2005
- Determination of Ascorbic Acid and Carotenoids in Food Commodities by Liquid Chromatography with Mass Spectrometry DetectionJournal of Agricultural and Food Chemistry, 2005
- Improved Normal-Phase High-Performance Liquid Chromatography Procedure for the Determination of Carotenoids in CerealsJournal of Agricultural and Food Chemistry, 2004
- Carotenoid Profiles and Consumer Sensory Evaluation of Specialty Carrots (Daucus carota, L.) of Various ColorsJournal of Agricultural and Food Chemistry, 2004
- Analysis of Xanthophylls in Corn by HPLCJournal of Agricultural and Food Chemistry, 2002
- Enzyme-Mediated Solvent Extraction of Carotenoids from Marigold Flower (Tagetes erecta)Journal of Agricultural and Food Chemistry, 2002
- Chromatographic determination of carotenoids in foodsJournal of Chromatography A, 2000
- SIMULTANEOUS QUANTIFICATION OF CAROTENOIDS AND TOCOPHEROLS IN CORN KERNEL EXTRACTS BY HPLCJournal of Liquid Chromatography & Related Technologies, 1999
- Analysis of carotenoids with emphasis on 9-cis β-carotene in vegetables and fruits commonly consumed in IsraelFood Chemistry, 1998
- Liquid chromatographic determination of carotenoids in human serum using an engineered C30 and a C18 stationary phaseJournal of Chromatography B: Biomedical Sciences and Applications, 1996