Application of Two-Dimensional Gel Electrophoresis To Interrogate Alterations in the Proteome of Genetically Modified Crops. 2. Assessing Natural Variability
- 16 February 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 54 (6), 2162-2168
- https://doi.org/10.1021/jf052357y
Abstract
Proteomics is currently tested as a complementary tool for the safety assessment of genetically modified (GM) crops. Understanding the natural variability of the proteome is crucial for the interpretation of biological differences between transgenic and nontransgenic parental lines. The natural variation of seed protein profiles among a set of 12 Arabidopsis thaliana ecotypes was determined by utilizing two-dimensional electrophoresis (2DE). The total number of different resolved protein spots found among the 12 ecotypes was 931 with a range of 573 (Mt-0) to 653 (Condara) in any one ecotype. Although the ecotypes were grown side-by-side in an environmentally controlled growth chamber, almost half of the resolved spots varied with respect to their presence/absence, and 95% of the spots present in all ecotypes varied in spot quantity (2−53-fold). In the evaluation of unintended effects of genetic modification, it is concluded that the experimental design must account for existing natural variability, which, in the case of the expressed proteome, can be substantial. Keywords: Two-dimensional gel electrophoresis; Arabidopsis thaliana; seed proteome; natural variabilityKeywords
This publication has 25 references indexed in Scilit:
- Proteome analysis differentiates between two highly homologues germin-like proteins in Arabidopsis thaliana ecotypes Col-0 and Ws-2Phytochemistry, 2004
- NATURALLY OCCURRING GENETIC VARIATION IN ARABIDOPSIS THALIANAAnnual Review of Plant Biology, 2004
- Aspects of the barley seed proteome during development and germinationBiochemical Society Transactions, 2004
- Genetic Control of Natural Variation in Arabidopsis Glucosinolate AccumulationPlant Physiology, 2001
- Quantification of silver‐stained proteins resolved by two‐dimensional electrophoresis: Genetic variability as related to abundance and solubility in two maize linesElectrophoresis, 1994
- Monitoring by two‐dimensional electrophoresis somatic embryogenesis in leaf and petiole explants from VitisElectrophoresis, 1992
- Quantitative analysis of two-dimensional protein profiles of inbred lines of maize (Zea mays L.)Electrophoresis, 1991
- Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250Electrophoresis, 1988
- The use of ultrathin-layer polyacrylamide gel isoelectric focusing in two-dimensional analysis of plant and fungal proteinsElectrophoresis, 1988
- Inheritance of Gliadin Proteins Associated with Quality in Durum Wheat1Crop Science, 1985