Label-free technologies for quantitative multiparameter biological analysis
- 17 February 2009
- journal article
- review article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 394 (1), 121-135
- https://doi.org/10.1007/s00216-009-2637-8
Abstract
In the postgenomic era, information is king and information-rich technologies are critically important drivers in both fundamental biology and medicine. It is now known that single-parameter measurements provide only limited detail and that quantitation of multiple biomolecular signatures can more fully illuminate complex biological function. Label-free technologies have recently attracted significant interest for sensitive and quantitative multiparameter analysis of biological systems. There are several different classes of label-free sensors that are currently being developed both in academia and in industry. In this critical review, we highlight, compare, and contrast some of the more promising approaches. We describe the fundamental principles of these different methods and discuss advantages and disadvantages that might potentially help one in selecting the appropriate technology for a given bioanalytical application.Keywords
This publication has 132 references indexed in Scilit:
- Effect of Fluorescently Labeling Protein Probes on Kinetics of Protein−Ligand ReactionsLangmuir, 2008
- Detection of human proteins using arrayed imaging reflectometryBiosensors and Bioelectronics, 2008
- Metallic Nanohole Arrays on Fluoropolymer Substrates as Small Label-Free Real-Time BioprobesNano Letters, 2008
- A General Method for Discovering Inhibitors of Protein−DNA Interactions Using Photonic Crystal BiosensorsACS Chemical Biology, 2008
- Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applicationsProceedings of the National Academy of Sciences of the United States of America, 2008
- Label-free flow-enhanced specific detection of Bacillus anthracis using a piezoelectric microcantilever sensorThe Analyst, 2008
- Electrical protein detection in cell lysates using high-density peptide-aptamer microarraysJournal of Biology, 2008
- Importance of the Debye Screening Length on Nanowire Field Effect Transistor SensorsNano Letters, 2007
- Label‐Free Impedance Biosensors: Opportunities and ChallengesElectroanalysis, 2007
- Thirty Years of CHEMFETs – A Personal ViewElectroanalysis, 2004