Practical Application of Thin-Layer Chromatography for Detection of Polyphosphates in Seafood

Abstract

The practical application of previously published thin-layer chromatographic (TLC) methods to polyphosphate detection in seafood was determined. Exogenous polyphosphates are distinguishable from naturally occurring phosphates by using TLC; their detection can be enhanced by sampling techniques. Polyphosphates will eventually hydrolyze to monophosphates and make detection virtually impossible. Hydrolysis and therefore, nondetection, is accelerated by certain commercial practices. Results indicate that TLC detection of added phosphates can be used as an inspection tool for fresh products and for frozen products stored about 1 year.