Three new acylated chalcone glucosides [okanin 4′-O-β-D-(4″-acetyl-6″-trans-p-coumaroyl)-glucoside, okanin 4′-O-β-D-(2″,4″-diacetyl-6″-trans-p-coumaroyl)-glucoside, and okanin 4′-O-β-D-(3″,4″-diacetyl-6″-trans-p-coumaroyl)-glucoside] have been isolated from the leaves of Bidens pilosa L. (Asteraceae). Their structures have been elucidated by spectroscopic methods (1H-NMR, 2D-1H-NMR, 13C-NMR spectroscopy).