Ligament Tissue Engineering Using Synthetic Biodegradable Fiber Scaffolds
- 1 October 1999
- journal article
- Published by Mary Ann Liebert Inc in Tissue Engineering
- Vol. 5 (5), 443-451
- https://doi.org/10.1089/ten.1999.5.443
Abstract
Tissue engineering offers the possibility of replacing damaged human ligaments with engineered ligament tissues. Hence, we attempted to culture in vitro ligament tissues by seeding human anterior cruciate ligament (ACL) and medial collateral ligament (MCL) cells onto synthetic biodegradable polymer fiber scaffolds. The ACL and MCL cells readily attached to the scaffold fibers. These cells and their secreted matrix soon surrounded the scaffold fibers and bridged the gaps in between. Beginning at 2 weeks, portions of the scaffolds were completely filled with tissue matrix. By 5 weeks, the scaffolds became single bundles of tissue. Thus the cell/fiber system appears to be a viable system for culturing ligament tissues. Additionally, cell proliferation under mechanical and biochemical stimuli was studied for up to 4 days. Whereas mechanical stimulus and transforming growth factor enhanced proliferation, inflammatory agents (lipopolysaccharide and complement C5a) had a negative effect. This work can thus contribute to a sound strategy for culturing replacement ligament tissues in vitro.Keywords
This publication has 25 references indexed in Scilit:
- Migration and healing of ligament cells under inflammatory conditionsJournal of Orthopaedic Research, 1997
- Signal pathways and ligament cell adhesivenessJournal of Orthopaedic Research, 1996
- Proliferative and matrix synthesis response of canine anterior cruciate ligament fibroblasts submitted to combined growth factorsJournal of Orthopaedic Research, 1996
- Adhesiveness of human ligament fibroblasts to lamininJournal of Orthopaedic Research, 1995
- Adhesion Strength of Human Ligament FibroblastsJournal of Biomechanical Engineering, 1994
- Tissue EngineeringScience, 1993
- Anterior cruciate ligament reconstruction using a composite collagenous prosthesisThe American Journal of Sports Medicine, 1992
- A partially biodegradable material device for repair and reconstruction of injured tendonsThe American Journal of Sports Medicine, 1985
- Five??? to Ten-year Follow-up Evaluation after Reconstruction of the Anterior Cruciate LigamentClinical Orthopaedics and Related Research, 1984
- Results of Use of the Central One-Third of the Patellar Ligament to Compensate for Anterior Cruciate Ligament DeficiencyClinical Orthopaedics and Related Research, 1980