Enhanced Healing of Diabetic Wounds by Topical Administration of Adipose Tissue-Derived Stromal Cells Overexpressing Stromal-Derived Factor-1: Biodistribution and Engraftment Analysis by Bioluminescent Imaging
Open Access
- 1 January 2011
- journal article
- research article
- Published by Hindawi Limited in Stem Cells International
- Vol. 2011, 1-11
- https://doi.org/10.4061/2011/304562
Abstract
Chronic ulcers represent a major health problem in diabetic patients resulting in pain and discomfort. Conventional therapy does not guarantee adequate wound repair. In diabetes, impaired healing is partly due to poor endothelial progenitor cells mobilisation and homing, with altered levels of the chemokine stromal-derived factor-1 (SDF-1) at the wound site. Adipose tissue-associated stromal cells (AT-SCs) can provide an accessible source of progenitor cells secreting proangiogenic factors and differentiating into endothelial-like cells. We demonstrated that topical administration of AT-SCs genetically modifiedex vivoto overexpress SDF-1, promotes wound healing into diabetic mice. In particular, byin vivobioluminescent imaging analysis, we monitored biodistribution and survival after transplantation of luciferase-expressing cells. In conclusion, this study indicates the therapeutic potential of AT-SCs administration in wound healing, through cell differentiation, enhanced cellular recruitment at the wound site, and paracrine effects associated with local growth-factors production.Keywords
Funding Information
- European Union (FP6 STREP LSHB-CT-2005-512102, RF 351467, HL099117, EB009689)
This publication has 42 references indexed in Scilit:
- The Role of Hypoxia in Bone Marrow–Derived Mesenchymal Stem Cells: Considerations for Regenerative Medicine ApproachesTissue Engineering, Part B: Reviews, 2010
- Unresolved issues in the management of ulcers of the foot in diabetesDiabetic Medicine, 2008
- Diabetic impairments in NO-mediated endothelial progenitor cell mobilization and homing are reversed by hyperoxia and SDF-1αJCI Insight, 2007
- Recombinant human platelet-derived growth factor enhanced dermal wound healing by a pathway involving ERK and c-fos in diabetic ratsJournal of Dermatological Science, 2007
- Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose TissueThe International Journal of Cell Cloning, 2006
- Stromal-derived factor-1 promotes the growth, survival, and development of human bone marrow stromal stem cellsBlood, 2005
- Improvement of Postnatal Neovascularization by Human Adipose Tissue-Derived Stem CellsCirculation, 2004
- Secretion of Angiogenic and Antiapoptotic Factors by Human Adipose Stromal CellsCirculation, 2004
- Human Adipose Tissue Is a Source of Multipotent Stem CellsMolecular Biology of the Cell, 2002
- A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor.1992