l-Rhamnose Antigen: A Promising Alternative to α-Gal for Cancer Immunotherapies
- 2 November 2010
- journal article
- Published by American Chemical Society (ACS) in ACS Chemical Biology
- Vol. 6 (2), 185-191
- https://doi.org/10.1021/cb100318z
Abstract
The targeting of autologous vaccines toward antigen presenting cells (APCs) via the in vivo complexation between anti α-Gal (anti-Gal) antibodies and α-Gal antigens presents a promising cancer immunotherapy with enhanced immunogenicity. This strategy takes advantage of the ubiquitous anti-Gal antibody in human serum. In contrast to the α-Gal epitope, the recent identification of high titers of anti-l-rhamnose (anti-Rha) antibodies in humans reveals a new approach toward immunotherapy employing l-rhamnose (Rha) monosaccharides. In order to evaluate this simple antigen in preclinical applications, we have synthesized Rha-conjugated immunogens and successfully induced high titers of anti-Rha antibodies in wildtype mice. Moreover, our studies demonstrate for the first time that wildtype mice could replace α1,3galactosyltransferase knockout (α1,3GT KO) mice in such antigen/antibody-mediated vaccine design when developing cancer immunotherapies.Keywords
This publication has 20 references indexed in Scilit:
- Anti-carbohydrate antibodies of normal sera: Findings, surprises and challengesMolecular Immunology, 2009
- Profiling Human Serum Antibodies with a Carbohydrate Antigen MicroarrayJournal of Proteome Research, 2009
- Mechanism for increased immunogenicity of vaccines that form in vivo immune complexes with the natural anti-Gal antibodyVaccine, 2009
- Glycan arrays: biological and medical applicationsCurrent Opinion in Chemical Biology, 2008
- The Galα1,3Galβ1,4GlcNAc-R (α-Gal) epitope: A carbohydrate of unique evolution and clinical relevanceBiochimica et Biophysica Acta (BBA) - General Subjects, 2008
- Protein microarrays to study carbohydrate-recognition eventsBioorganic & Medicinal Chemistry Letters, 2006
- Sweet spots in functional glycomicsNature Chemical Biology, 2006
- The α‐gal epitope and the anti‐Gal antibody in xenotransplantation and in cancer immunotherapyImmunology & Cell Biology, 2005
- Antibody–oligosaccharide interactions: the synthesis of 2-deoxy-α-L-rhamnose containing oligosaccharide haptens related to Shigella flexneri variant Y antigenCanadian Journal of Chemistry, 1993
- A unique natural human IgG antibody with anti-alpha-galactosyl specificity.The Journal of Experimental Medicine, 1984