Physiology and molecular genetics of poly(β‐hydroxyalkanoic acid) synthesis in Alcaligenes eutrophus
Open Access
- 1 March 1991
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 5 (3), 535-542
- https://doi.org/10.1111/j.1365-2958.1991.tb00725.x
Abstract
The Alcaligenes eutrophus genes for β-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase and poly(β-hydroxybutyric acid) synthase (PHB synthase) which comprise the three-step PHB-biosynthetic pathway, were cloned. Molecular studies revealed that these genes are organized in a single operon. The A. eutrophus PHB-biosynthetic genes are readily expressed in other bacteria, and DNA fragments harbouring the operon can be used as a cartridge to confer to other bacteria the ability to synthesize PHB from acetyl-CoA. The biochemical and physiological capabilities of A. eutrophus for the synthesis of a wide variety of polyhydroxyalkanoates are discussed.Keywords
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