A cold plasma jet accelerates wound healing in a murine model of full‐thickness skin wounds
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- 30 January 2017
- journal article
- research article
- Published by Wiley in Experimental Dermatology
- Vol. 26 (2), 156-162
- https://doi.org/10.1111/exd.13156
Abstract
Cold plasma has been successfully applied in several fields of medicine that require, for example, pathogen inactivation, implant functionalization or alteration of cellular activity. Previous studies have provided evidence that plasma supports the healing of wounds owing to its beneficial mixtures of reactive species and modulation of inflammation in cells and tissues. To investigate the wound healing activity of an atmospheric pressure plasma jet in vivo, we examined the cold plasma's efficacy on dermal regeneration in a murine model of dermal full-thickness ear wound. Over 14 days, female mice received daily plasma treatment. Quantitative analysis by transmitted light microscopy demonstrated a significantly accelerated wound re-epithelialization at days 3–9 in comparison with untreated controls. In vitro, cold plasma altered keratinocyte and fibroblast migration, while both cell types showed significant stimulation resulting in accelerated closure of gaps in scratch assays. This plasma effect correlated with the downregulation of the gap junctional protein connexin 43 which is thought to be important in the regulation of wound healing. In addition, plasma induced profound changes in adherence junctions and cytoskeletal dynamics as shown by downregulation of E-cadherin and several integrins as well as actin reorganization. Our results theorize cold plasma to be a beneficial treatment option supplementing existing wound therapies.Keywords
Funding Information
- European Social Fund (AU 11 038, ESF/IV‐BM‐B35‐0010/13, AU 15 001)
- Bundesministerium für Bildung und Forschung (03Z22DN11)
This publication has 85 references indexed in Scilit:
- Cold Atmospheric Plasma (CAP) Changes Gene Expression of Key Molecules of the Wound Healing Machinery and Improves Wound Healing In Vitro and In VivoPLOS ONE, 2013
- Differential Influence of Components Resulting from Atmospheric-Pressure Plasma on Integrin Expression of Human HaCaT KeratinocytesBioMed Research International, 2013
- Molecular Crosstalk between Integrins and Cadherins: Do Reactive Oxygen Species Set the Talk?Journal of Signal Transduction, 2011
- Human skin wounds: A major and snowballing threat to public health and the economyWound Repair and Regeneration, 2009
- Connexin43 Carboxyl-Terminal Peptides Reduce Scar Progenitor and Promote Regenerative Healing Following Skin WoundingRegenerative Medicine, 2009
- Loss of Integrin α9β1 Results in Defects in Proliferation, Causing Poor Re-Epithelialization during Cutaneous Wound HealingJournal of Investigative Dermatology, 2009
- Redox signals in wound healingBiochimica et Biophysica Acta (BBA) - General Subjects, 2008
- In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitroNature Protocols, 2007
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Integrin-ligand binding properties govern cell migration speed through cell-substratum adhesivenessNature, 1997