Selective saturation of slow endocytosis at a giant glutamatergic central synapse lacking dynamin 1
Open Access
- 11 November 2008
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 105 (45), 17555-17560
- https://doi.org/10.1073/pnas.0809621105
Abstract
Exocytosis of synaptic vesicles is rapidly followed by compensatory plasma membrane endocytosis. The efficiency of endocytosis varies with experimental conditions, but the molecular basis for this control remains poorly understood. Here, the function of dynamin 1, the neuron-specific member of a family of GTPases implicated in vesicle fission, was investigated with high temporal resolution via membrane capacitance measurements at the calyx of Held, a giant glutamatergic synapse. Endocytosis at dynamin 1 KO calyces was the same as in wild type after weak stimuli, consistent with the nearly normal ultrastructure of mutant synapses. However, following stronger stimuli, the speed of slow endocytosis, but not of other forms of endocytosis, failed to scale with the increased endocytic load. Thus, high level expression of dynamin 1 is essential to allow the slow, clathrin-mediated endocytosis, which accounts for the bulk of the endocytic response, to operate efficiently over a wide range of activity.Keywords
This publication has 36 references indexed in Scilit:
- Clathrin Dependence of Synaptic-Vesicle Formation at the Drosophila Neuromuscular JunctionCurrent Biology, 2008
- Cell- and stimulus-dependent heterogeneity of synaptic vesicle endocytic recycling mechanisms revealed by studies of dynamin 1-null neuronsProceedings of the National Academy of Sciences of the United States of America, 2008
- Single-vesicle imaging reveals that synaptic vesicle exocytosis and endocytosis are coupled by a single stochastic modeProceedings of the National Academy of Sciences, 2007
- Posttetanic potentiation critically depends on an enhanced Ca 2+ sensitivity of vesicle fusion mediated by presynaptic PKCProceedings of the National Academy of Sciences of the United States of America, 2007
- Rapid bulk endocytosis and its kinetics of fission pore closure at a central synapseProceedings of the National Academy of Sciences, 2007
- Inhibition of dynamin completely blocks compensatory synaptic vesicle endocytosisProceedings of the National Academy of Sciences, 2006
- Clathrin-Mediated Endocytosis Is the Dominant Mechanism of Vesicle Retrieval at Hippocampal SynapsesNeuron, 2006
- Clathrin-Dependent and Clathrin-Independent Retrieval of Synaptic Vesicles in Retinal Bipolar CellsNeuron, 2005
- Synaptic Vesicle Endocytosis Impaired by Disruption of Dynamin-SH3 Domain InteractionsScience, 1997
- EVIDENCE FOR RECYCLING OF SYNAPTIC VESICLE MEMBRANE DURING TRANSMITTER RELEASE AT THE FROG NEUROMUSCULAR JUNCTIONThe Journal of cell biology, 1973