Evidence for structural differences between human glucose-6-phosphate dehydrogenase purified from leukocytes and erythrocytes
- 11 July 1977
- journal article
- research article
- Published by Elsevier BV in Biochemical and Biophysical Research Communications
- Vol. 77 (1), 65-72
- https://doi.org/10.1016/s0006-291x(77)80165-4
Abstract
The C-terminal end of pure glucose-6-phosphate dehydrogenase from human leukocytes and red cells has been determined by subjecting these enzymes to partial proteolysis by carboxypeptidase A and B. The C-termini of the leukocyte enzyme were Lysine-Leucine, while Glycine was found as the C-terminal residue of erythrocyte glucose-6-phosphate dehydrogenase. From the results reported in this paper and from data previously reported we may conclude that aging of glucose-6-phosphate dehydrogenase in the red cells is associated with partial proteolysis of the C-terminal end of the enzyme molecules.Keywords
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