Separation and parallel sequencing of the genomes and transcriptomes of single cells using G&T-seq
- 29 September 2016
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Protocols
- Vol. 11 (11), 2081-2103
- https://doi.org/10.1038/nprot.2016.138
Abstract
G&T-seq enables sequencing of DNA and mRNA from the same single cell, allowing the effects of genomic variation on transcription to be studied. It is compatible with any WGA method and so can be tailored to specific applications.This publication has 51 references indexed in Scilit:
- TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusionsGenome Biology, 2013
- Single-Neuron Sequencing Analysis of L1 Retrotransposition and Somatic Mutation in the Human BrainCell, 2012
- Genome-wide Single-Cell Analysis of Recombination Activity and De Novo Mutation Rates in Human SpermCell, 2012
- Single-Cell Exome Sequencing Reveals Single-Nucleotide Mutation Characteristics of a Kidney TumorCell, 2012
- Direct, genome-wide assessment of DNA mutations in single cellsNucleic Acids Research, 2011
- A framework for variation discovery and genotyping using next-generation DNA sequencing dataNature Genetics, 2011
- Fast and accurate long-read alignment with Burrows–Wheeler transformBioinformatics, 2010
- The Sequence Alignment/Map format and SAMtoolsBioinformatics, 2009
- Fast and accurate short read alignment with Burrows–Wheeler transformBioinformatics, 2009
- The International HapMap ProjectNature, 2003