Detection, Isolation, and Identification of Vibrio cholerae from the Environment
Open Access
- 1 August 2012
- journal article
- unit
- Published by Wiley in Current Protocols in Microbiology
- Vol. 26 (1), 6A.5.1-6A.5.51
- https://doi.org/10.1002/9780471729259.mc06a05s26
Abstract
Recent molecular advances in microbiology have greatly improved the detection of bacterial pathogens in the environment. These improvements and a downward trend in the cost of molecular detection methods have contributed to increased frequency of detection of pathogenic microorganisms where traditional culture‐based detection methods have failed. Culture methods also have been greatly improved, and the confluence of the two suites of methods provides a powerful tool for detection, isolation, and characterization of pathogens. While molecular detection provides data on the presence and type of pathogens, culturing methods allow a researcher to preserve the organism of interest for “‐omics” studies, such as genomic, metabolomic, secretomic, and transcriptomic analysis, which are rapidly becoming more affordable. This has yielded a clearer understanding of the ecology and epidemiology of microorganisms that cause disease. In this unit, we present commonly accepted methods for isolation, detection, and characterization of V. cholerae, providing more extensive knowledge of the ecology and epidemiology of this organism. This unit has been fully revised and updated from the earlier version with the latest knowledge and additional information not previously included. Curr. Protoc. Microbiol. 26:6A.5.1‐6A.5.51. © 2012 by John Wiley & Sons, Inc.Keywords
This publication has 91 references indexed in Scilit:
- Ecology and Genetic Structure of a Northern Temperate Vibrio cholerae Population Related to Toxigenic IsolatesApplied and Environmental Microbiology, 2011
- Discovery of novel Vibrio cholerae VSP-II genomic islands using comparative genomic analysisFEMS Microbiology Letters, 2010
- Incidence, Virulence Factors, and Clonality among Clinical Strains of Non-O1, Non-O139 Vibrio cholerae Isolates from Hospitalized Diarrheal Patients in Kolkata, IndiaJournal of Clinical Microbiology, 2009
- Environmental signatures associated with cholera epidemicsProceedings of the National Academy of Sciences of the United States of America, 2008
- Association of Vibrio cholerae O1 El Tor and O139 Bengal with the Copepods Acartia tonsa and Eurytemora affinisApplied and Environmental Microbiology, 2007
- Molecular-Beacon Multiplex Real-Time PCR Assay for Detection of Vibrio choleraeApplied and Environmental Microbiology, 2006
- Seasonal Cholera Caused by Vibrio cholerae Serogroups O1 and O139 in the Coastal Aquatic Environment of BangladeshApplied and Environmental Microbiology, 2006
- Predictability of Vibrio cholerae in Chesapeake BayApplied and Environmental Microbiology, 2003
- Genotypes Associated with Virulence in Environmental Isolates of Vibrio choleraeApplied and Environmental Microbiology, 2001
- Vibrio cholerae produces a second enterotoxin, which affects intestinal tight junctions.Proceedings of the National Academy of Sciences of the United States of America, 1991