Rhizobium strains competitiveness on bean nodulation in Cerrado soils
Open Access
- 1 March 2006
- journal article
- Published by FapUNIFESP (SciELO) in Pesquisa Agropecuária Brasileira
- Vol. 41 (3), 439-447
- https://doi.org/10.1590/s0100-204x2006000300010
Abstract
The objective of this work was to identify the most competitive and effective Rhizobium strains in order to increase common bean yield by nitrogen fixation as alternative or complementation to the nitrogen fertilization. Competitiveness tests were lead in axenic conditions, in Cerrado soil pots and in three field experiments, with native Rhizobium strains that were previously identified, according to their effectiveness and genetic variability. The identification of strains in nodules was performed using serological tests (axenic conditions) - agglutination and enzyme linked immunosorbent (Elisa) assays - and random amplified polymorfic DNA (RAPD) (Cerrado soil). Plant yield was determined using the dry weight (greenhouse conditions), total N and grain yield (field experiments). Among the analyzed Rhizobium strains, native strain SLA 2.2 and commercial strain CIAT 899 were the dominant nodules in plants of the most productive plots, presenting yield productivity similar or higher to those obtained in treatments where 20 kg ha-1 of N were applied.Keywords
This publication has 13 references indexed in Scilit:
- Competition between strains of Bradyrhizobium japonicum for nodulation of soybeans in Argentine arable soilsApplied Soil Ecology, 1999
- Importance of host plants for detecting the population diversity of Rhizobium leguminosarum biovar viciae in soilSoil Biology and Biochemistry, 1998
- Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris NodulesInternational Journal of Systematic and Evolutionary Microbiology, 1997
- Effects of common bean (Phaseolus vulgaris L.) cultivar and rhizobium strain on plant growth, seed yield and nitrogen contentCanadian Journal of Plant Science, 1997
- Genomic heterogeneity within bean nodulating Rhizobium strains isolated from cerrado soilsSoil Biology and Biochemistry, 1997
- Competitive interaction among strains of Rhizobium leguminosarum bv. phaseoli in the nodulation of kidney beans (Phaseolus vulgaris L.)Biology and Fertility of Soils, 1996
- The contributions of plant and bacteria genotypes in the growth and nitrogen accumulation of inoculated alfalfaPlant and Soil, 1994
- Genetic and population study of a Y-linked tetranucleotide repeat DNA polymorphism with a simple non-isotopic techniqueHuman Genetics, 1993
- Rhizobium tropici, a Novel Species Nodulating Phaseolus vulgaris L. Beans and Leucaena sp. TreesInternational Journal of Systematic and Evolutionary Microbiology, 1991
- Examining serological diversity of ?cowpea? rhizobia by the ELISA techniqueArchiv für Mikrobiologie, 1981