Proteomic Analysis of MeJa-Induced Defense Responses in Rice against Wounding
Open Access
- 22 May 2019
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 20 (10), 2525
- https://doi.org/10.3390/ijms20102525
Abstract
The role of jasmonates in defense priming has been widely recognized. Priming is a physiological process by which a plant exposed to low doses of biotic or abiotic elicitors activates faster and/or stronger defense responses when subsequently challenged by a stress. In this work, we investigated the impact of MeJA-induced defense responses to mechanical wounding in rice (Oryza sativa). The proteome reprogramming of plants treated with MeJA, wounding or MeJA+wounding has been in-depth analyzed by using a combination of high throughput profiling techniques and bioinformatics tools. Gene Ontology analysis identified protein classes as defense/immunity proteins, hydrolases and oxidoreductases differentially enriched by the three treatments, although with different amplitude. Remarkably, proteins involved in photosynthesis or oxidative stress were significantly affected upon wounding in MeJA-primed plants. Although these identified proteins had been previously shown to play a role in defense responses, our study revealed that they are specifically associated with MeJA-priming. Additionally, we also showed that at the phenotypic level MeJA protects plants from oxidative stress and photosynthetic damage induced by wounding. Taken together, our results add novel insight into the molecular actors and physiological mechanisms orchestrated by MeJA in enhancing rice plants defenses after wounding.Keywords
This publication has 126 references indexed in Scilit:
- Hyperosmotic priming of Arabidopsis seedlings establishes a long-term somatic memory accompanied by specific changes of the epigenomeGenome Biology, 2013
- The use of metabolomics to dissect plant responses to abiotic stressesCellular and Molecular Life Sciences, 2012
- Class III peroxidases in plant defence reactionsJournal of Experimental Botany, 2008
- MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantificationNature Biotechnology, 2008
- Characteristic expression of twelve rice PR1 family genes in response to pathogen infection, wounding, and defense-related signal compounds (121/180)Molecular Genetics and Genomics, 2008
- Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cellsProceedings of the National Academy of Sciences of the United States of America, 2008
- The plant immune systemNature, 2006
- Fragments of ATP synthase mediate plant perception of insect attackProceedings of the National Academy of Sciences of the United States of America, 2006
- Structure of the cinnamyl-alcohol dehydrogenase gene family in rice and promoter activity of a member associated with lignificationPlanta, 2004
- Catalases in plantsMolecular Breeding, 1995