Comparative analysis of the expression of ERBIN and Erb-B2 in normal human skin and cutaneous carcinomas.
- 22 May 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in British Journal of Dermatology
- Vol. 152 (6), 1248-1255
- https://doi.org/10.1111/j.1365-2133.2005.06687.x
Abstract
Background ERBIN is a binding partner of Erb‐B2, an orphan receptor within the Erb‐B family critically involved in the regulation of cell growth and differentiation. Although its function remains unclear, ERBIN is thought to affect the polarity of epithelial cells and cell growth via the Ras signalling pathway. Objectives To examine and compare the tissue distribution and the expression levels of ERBIN and Erb‐B2 in normal skin and in cutaneous carcinomas. Methods Fifteen cases of basal cell carcinoma (BCC), 12 cases of squamous cell carcinoma (SCC) and five cases of keratoacanthoma (KA) were analysed by immunohistochemistry on paraffin‐embedded sections using anti‐ERBIN and anti‐Erb‐B2 antibodies. Results ERBIN and Erb‐B2 had a similar distribution in normal human skin. They were primarily localized at the plasma membrane in differentiated keratinocytes and in duct cells from eccrine glands, whereas they were localized diffusely in the cytoplasma of basal keratinocytes. In both SCC and KA the subcellular distribution of ERBIN and Erb‐B2 remained unchanged, whereas both proteins were redistributed from the plasma membrane into cytosolic aggregates in BCC. Conclusions The subcellular localization of ERBIN in normal human skin is similar to that of Erb‐B2 and varies with cell differentiation. Based on our findings and on the biological activities of Erb‐B2, it is conceivable that disturbed expression or functioning of ERBIN and Erb‐B2 is implicated in the development of the malignant phenotype of BCC.Keywords
This publication has 23 references indexed in Scilit:
- Lano, a Novel LAP Protein Directly Connected to MAGUK Proteins in Epithelial CellsPublished by Elsevier BV ,2001
- Erbin Is a Protein Concentrated at Postsynaptic Membranes That Interacts with PSD-95Published by Elsevier BV ,2001
- The ERBB2/HER2 Receptor Differentially Interacts with ERBIN and PICK1 PSD-95/DLG/ZO-1 Domain ProteinsPublished by Elsevier BV ,2001
- NEW EMBO MEMBERS' REVIEW: The ErbB signaling network: receptor heterodimerization in development and cancerThe EMBO Journal, 2000
- Transgenic expression of the human amphiregulin gene induces a psoriasis-like phenotype.JCI Insight, 1997
- Increased level of c-erbB-2/neu/HER-2 protein in cutaneous squamous cell carcinomaBritish Journal of Dermatology, 1997
- Polarized Expression of HD1: Relationship with the Cytoskeleton in Cultured Human Colonic Carcinoma CellsExperimental Cell Research, 1997
- Expression of α6β4 integrin increases during malignant conversion of mouse epidermal keratinocytes: Association of β4 subunit to the cytokeratin fractionExperimental Cell Research, 1992
- Differential Expression of Laminin Isoforms and β4 Integrin Epitopes in the Basement Membrane Zone of Normal Human Skin and Basal Cell CarcinomasJournal of Investigative Dermatology, 1992
- A specific antigenic defect of the basement membrane is found in basal cell carcinoma but not in other epidermal tumorsCancer, 1982