In vitro neurogenesis: development and functional implications of iPSC technology
- 20 November 2013
- journal article
- review article
- Published by Springer Science and Business Media LLC in Cellular and Molecular Life Sciences
- Vol. 71 (9), 1623-1639
- https://doi.org/10.1007/s00018-013-1511-1
Abstract
Neurogenesis is the developmental process regulating cell proliferation of neural stem cells, determining their differentiation into glial and neuronal cells, and orchestrating their organization into finely regulated functional networks. Can this complex process be recapitulated in vitro using induced pluripotent stem cell (iPSC) technology? Can neurodevelopmental and neurodegenerative diseases be modeled using iPSCs? What is the potential of iPSC technology in neurobiology? What are the recent advances in the field of neurological diseases? Since the applications of iPSCs in neurobiology are based on the capacity to regulate in vitro differentiation of human iPSCs into different neuronal subtypes and glial cells, and the possibility of obtaining iPSC-derived neurons and glial cells is based on and hindered by our poor understanding of human embryonic development, we reviewed current knowledge on in vitro neural differentiation from a developmental and cellular biology perspective. We highlight the importance to further advance our understanding on the mechanisms controlling in vivo neurogenesis in order to efficiently guide neurogenesis in vitro for cell modeling and therapeutical applications of iPSCs technology.Keywords
This publication has 119 references indexed in Scilit:
- EZ spheres: A stable and expandable culture system for the generation of pre-rosette multipotent stem cells from human ESCs and iPSCsStem Cell Research, 2013
- Induced Pluripotent Stem Cell-Derived Neural Cells Survive and Mature in the Nonhuman Primate BrainCell Reports, 2013
- All-trans-retinoid acid induces the differentiation of encapsulated mouse embryonic stem cells into GABAergic neuronsDifferentiation, 2012
- RETRACTED: Directed Conversion of Alzheimer's Disease Patient Skin Fibroblasts into Functional NeuronsCell, 2011
- Generation of endoderm-derived human induced pluripotent stem cells from primary hepatocytesHepatology, 2010
- Molecular and phenotypic reassessment of an infrequently used mouse model for spinal muscular atrophyBiochemical and Biophysical Research Communications, 2010
- FMR1: A gene with three facesBiochimica et Biophysica Acta (BBA) - General Subjects, 2009
- Developmental Study of Fragile X Syndrome Using Human Embryonic Stem Cells Derived from Preimplantation Genetically Diagnosed EmbryosCell Stem Cell, 2007
- Mecp2 deficiency leads to delayed maturation and altered gene expression in hippocampal neuronsNeurobiology of Disease, 2007
- Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined FactorsCell, 2006