Mobilization of Endothelial Progenitor Cells in Patients With Acute Myocardial Infarction
Open Access
- 12 June 2001
- journal article
- clinical trial
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation
- Vol. 103 (23), 2776-2779
- https://doi.org/10.1161/hc2301.092122
Abstract
Background —Endothelial progenitor cells (EPCs) circulate in adult peripheral blood (PB) and contribute to neovascularization. However, little is known regarding whether EPCs and their putative precursor, CD34-positive mononuclear cells (MNC CD34+ ), are mobilized into PB in acute ischemic events in humans. Methods and Results —Flow cytometry revealed that circulating MNC CD34+ counts significantly increased in patients with acute myocardial infarction (n=16), peaking on day 7 after onset, whereas they were unchanged in control subjects (n=8) who had no evidence of cardiac ischemia. During culture, PB-MNCs formed multiple cell clusters, and EPC-like attaching cells with endothelial cell lineage markers (CD31, vascular endothelial cadherin, and kinase insert domain receptor) sprouted from clusters. In patients with acute myocardial infarction, more cell clusters and EPCs developed from cultured PB-MNCs obtained on day 7 than those on day 1. Plasma levels of vascular endothelial growth factor significantly increased, peaking on day 7, and they positively correlated with circulating MNC CD34+ counts ( r =0.35, P =0.01). Conclusions —This is the first clinical demonstration showing that lineage-committed EPCs and MNC CD34+ , their putative precursors, are mobilized during an acute ischemic event in humans.Keywords
This publication has 10 references indexed in Scilit:
- VEGF gene transfer mobilizes endothelial progenitor cells in patients with inoperable coronary diseaseThe Annals of Thoracic Surgery, 2000
- Transplanted cord blood–derived endothelial precursor cells augment postnatal neovascularizationJCI Insight, 2000
- Evidence from a leukaemia model for maintenance of vascular endothelium by bone-marrow-derived endothelial cellsThe Lancet, 2000
- Transplantation ofex vivoexpanded endothelial progenitor cells for therapeutic neovascularizationProceedings of the National Academy of Sciences of the United States of America, 2000
- Early Expression of Angiogenesis Factors in Acute Myocardial Ischemia and InfarctionNew England Journal of Medicine, 2000
- Origins of circulating endothelial cells and endothelial outgrowth from bloodJCI Insight, 2000
- VEGF contributes to postnatal neovascularization by mobilizing bone marrow-derived endothelial progenitor cellsThe EMBO Journal, 1999
- Angiogenesis and vasculogenesis as therapeutic strategies for postnatal neovascularizationJCI Insight, 1999
- Direct Evidence of Endothelial Injury in Acute Myocardial Infarction and Unstable Angina by Demonstration of Circulating Endothelial CellsBlood, 1999
- Isolation of Putative Progenitor Endothelial Cells for AngiogenesisScience, 1997