Measurement of serum testosterone using high-performance liquid chromatography/tandem mass spectrometry
- 1 January 2006
- journal article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 44 (1), 70-5
- https://doi.org/10.1515/cclm.2006.014
Abstract
Low levels of serum testosterone typically found in women and children cannot be reliably measured by immunoassay. We developed a simple and sensitive method using high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS). Sample preparation involved protein precipitation of serum (1.0mL) with acetonitrile containing the internal standard (testosterone-Keywords
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