Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.
Open Access
- 1 March 1984
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 98 (3), 922-933
- https://doi.org/10.1083/jcb.98.3.922
Abstract
The distribution of actin and tubulin during the cell cycle of the budding yeast Saccharomyces was mapped by immunofluorescence using fixed cells from which the walls had been removed by digestion. The intranuclear mitotic spindle was shown clearly by staining with a monoclonal antitubulin; the presence of extensive bundles of cytoplasmic microtubules is reported. In cells containing short spindles still entirely within the mother cells, one of the bundles of cytoplasmic microtubules nearly always extended to (or into) the bud. Two independent reagents (anti-yeast actin and fluorescent phalloidin) revealed an unusual distribution of actin: it was present as a set of cortical dots or patches and also as distinct fibers that were presumably bundles of actin filaments. Double labeling showed that at no stage in the cell cycle do the distributions of actin and tubulin coincide for any significant length, and, in particular, that the mitotic spindle did not stain detectably for actin. However, both microtubule and actin staining patterns change in a characteristic way during the cell cycle. In particular, the actin dots clustered in rings about the bases of very small buds and at the sites on unbudded cells at which bud emergence was apparently imminent. Later in the budding cycle, the actin dots were present largely in the buds and, in many strains, primarily at the tips of these buds. At about the time of cytokinesis the actin dots clustered in the neck region between the separating cells. These aspects of actin distribution suggest that it may have a role in the localized deposition of new cell wall material.Keywords
This publication has 49 references indexed in Scilit:
- Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.The Journal of cell biology, 1982
- Isolation and characterization of a proteinaceous subnuclear fraction composed of nuclear matrix, peripheral lamina, and nuclear pore complexes from embryos of Drosophila melanogaster.The Journal of cell biology, 1982
- A simple method of reducing the fading of immunofluorescence during microscopyJournal of Immunological Methods, 1981
- Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.Journal of Histochemistry & Cytochemistry, 1981
- Early changes in the distribution and organization of microfilament proteins during cell transformationCell, 1981
- Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America, 1980
- Replacement of chromosome segments with altered DNA sequences constructed in vitro.Proceedings of the National Academy of Sciences of the United States of America, 1979
- Duplication of Spindle Plaques and Integration of the Yeast Cell CyclePublished by Cold Spring Harbor Laboratory ,1974
- A rapid method for desalting small volumes of solutionAnalytical Biochemistry, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970