In Situ Growing Triethanolamine-Functionalized Metal–Organic Frameworks on Two-Dimensional Carbon Nanosheets for Electrochemiluminescent Immunoassay
- 26 August 2019
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Sensors
- Vol. 4 (9), 2351-2357
- https://doi.org/10.1021/acssensors.9b00914
Abstract
A facile strategy for in-situ growing triethanolamine (TEOA)-functionalized metal-organic framework ([email protected]) on the 2D graphene oxide (GO) or g-C3N4 nanosheets via the self-assembly technique was introduced. In this method, Zn2+ was firstly attached on the carbon nanosheets by electrostatic interaction, then trimesic acid (H3btc) acted as the complex agent and TEOA as a base for the deprotonation of H3btc and a template, which leads to in situ growing the MOF on the carbon nanosheets obtaining a sandwich-like structure. Different types of surface analysis techniques were employed to characterize the [email protected] and [email protected] nanomaterials fabricated. The GO-TEOA[email protected] or [email protected] nanomaterials modified electrode brings out obviously enhanced ECL behaviors due to numerous TEOA in the framework structures. Specifically, both TEOA and GO can serve as the co-reactants for ECL system of Ru(bpy)32+ and have the synergic effect of enhancing signal. Based on the [email protected] modified electrodes, we developed a sensitive and rapid label-free ECL immunoassay strategy for human copeptin, and the linear range was 5 pg mL-1 ‒500 ng mL-1 as well as the limit of detection was 360 fg mL-1. This work exhibits excellent specificity and good stability of prepared immunosensor in the practical sample determination, demonstrating it can serve as a very promising method for the clinical diagnostics of acute myocardial infarction (AMI) disease.Keywords
Funding Information
- Ministry of Science and Technology of the People's Republic of China (2016YFA0201300)
- China Postdoctoral Science Foundation (2016M600846)
- National Natural Science Foundation of China (21575006, 21335001)
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